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Functional Characterization of a Cystathionine beta-Synthase Gene in Sulfur Metabolism and Pathoge

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Functional Characterization of a Cystathionine beta-Synthase Gene in Sulfur Metabolism and Pathogenicity of Aspergillus niger in Pear Fruit
文献类型:期刊
通讯作者:Zhang, H; Hu, KD (reprint author), Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230009, Anhui, Peoples R China.
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY影响因子和分区
年:2019
卷:67
期:16
页码:4435-4443
ISSN:0021-8561
关键词:Aspergillus niger; cystathionine beta-synthase; sulfur metabolism; cadmium stress; pathogenicity
摘要:Aspergillus niger, which is a fungal pathogen, causes rot in a variety of fruits. In this study, the cystathionine beta-synthase cbsA gene was deleted by homologous recombination to study its role in sulfur metabolism and pathogenicity of A. niger. The results showed that Delta cbsA strain maintained normal mycelia growth and sporulation compared with the control strain A. niger MA 70.15, whereas the contents of cysteine and glutathione (GSH) increased significantly after cbsA deletion. However, ...More
Aspergillus niger, which is a fungal pathogen, causes rot in a variety of fruits. In this study, the cystathionine beta-synthase cbsA gene was deleted by homologous recombination to study its role in sulfur metabolism and pathogenicity of A. niger. The results showed that Delta cbsA strain maintained normal mycelia growth and sporulation compared with the control strain A. niger MA 70.15, whereas the contents of cysteine and glutathione (GSH) increased significantly after cbsA deletion. However, Delta cbsA strain showed reduced endogenous H2S production. Further results showed that cbsA gene deletion induced higher resistance to cadmium stress and stronger infectivity to pears. It was also found that a stronger response of reactive oxygen species (ROS) production was induced in Delta cbsA mutant-infected pear compared with the control strain. In all, the present research suggested the important role of cbsA in sulfur metabolism and pathogenicity of A. niger in pear fruit. ...Hide

DOI:10.1021/acs.jafc.9b00325
百度学术:Functional Characterization of a Cystathionine beta-Synthase Gene in Sulfur Metabolism and Pathogenicity of Aspergillus niger in Pear Fruit
语言:外文
人气指数:3
浏览次数:3
基金:National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31670278, 31470013, 31300133]; Anhui Provincial Science and Technology Major Project [16030701073]; Earmarked Fund for the China Agriculture Research System [CARS-10-B1]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [JZ2018HGTB0241, JZ2018HGBZ0160]; Natural Science Foundation of Anhui ProvinceNatural Science Foundation of Anhui Province [1908085MC72]; Key Research and Development Program of Anhui Province [201904a06020031]; National Undergraduate Training Programs for Innovation of China [201810359054, 2018CXCYS195]
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