文献详情
Enhancement of Lysozyme Crystallization Using DNA as a Polymeric Additive
文献类型：期刊
通讯作者：Yang, ZQ (reprint author), Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China.; Xu, LJ (reprint author), Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China.; Heng, JYY (reprint author), Imperial Coll London, Dept Chem Engn, SPEL, South Kensington Campus, London SW7 2AZ, England.
期刊名称：CRYSTALS影响因子和分区
年：2019
卷：9
期：4
ISSN：2073-4352
关键词：lysozyme crystallization; DNA; additive; volume exclusion
所属部门：化学系
摘要：This study reports the first experimental evidence of using DNA as a polymeric additive to enhance protein crystallization. Using three kinds of DNA with different molecular weightscalf DNA, salmon DNA, and herring DNAthis study showed an improvement in the success rate of lysozyme crystallization, as compared to control experiments, especially at low lysozyme concentration. The improvement of crystallization is particularly significant in the presence of calf DNA with the highest molecular weig ...More
This study reports the first experimental evidence of using DNA as a polymeric additive to enhance protein crystallization. Using three kinds of DNA with different molecular weightscalf DNA, salmon DNA, and herring DNAthis study showed an improvement in the success rate of lysozyme crystallization, as compared to control experiments, especially at low lysozyme concentration. The improvement of crystallization is particularly significant in the presence of calf DNA with the highest molecular weight. Calf DNA also speeds up the induction time of lysozyme crystallization and increases the number of crystals per drop. We hypothesized the effect of DNA on protein crystallization may be due to the combination of excluded volume effect, change of water's surface tension, and the water competition effect. This work confirms predications of the potential use of DNA as a polymeric additive to enhance protein crystallization, potentially applied to systems with limited protein available or difficult to crystallize. ...Hide

DOI：10.3390/cryst9040186
百度学术：Enhancement of Lysozyme Crystallization Using DNA as a Polymeric Additive
语言：外文
人气指数：2
浏览次数：2
基金：National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21474059, 21372258]; Royal Academy of Engineering-Research Exchange China and India (RECI) program [1314RECI047]; EPSRCEngineering & Physical Sciences Research Council (EPSRC) [EP/N015916/1]
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