文献详情
Nanoscale ATP-Responsive Zeolitic Imidazole Framework-90 as a General Platform for Cytosolic Protein Delivery and Genome Editing
文献类型：期刊
通讯作者：Wang, M; Mao, LQ (reprint author), Chinese Acad Sci, CAS Res Educ Ctr Excellence Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci,Inst Chem, Beijing 100190, Peoples R China.; Wang, M; Mao, LQ (reprint author), Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
期刊名称：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY影响因子和分区
年：2019
卷：141
期：9
页码：3782-3786
ISSN：0002-7863
所属部门：化学系
摘要：Metal-organic frameworks (MOFs) are an emerging class of nanocarriers for drug delivery, owing to their tunable chemical functionality. Here we report ATP-responsive zeolitic imidazole framework-90 (ZIF-90) as a general platform for cytosolic protein delivery and CRISPR/Cas9 genome editing. The self-assembly of imidazole-2-carboxaldehyde and Zn2+ with protein forms ZIF-90/protein nanoparticles and efficiently encapsulates protein. It was found that, in the presence of ATP, the ZIF-90/protein nan ...More
Metal-organic frameworks (MOFs) are an emerging class of nanocarriers for drug delivery, owing to their tunable chemical functionality. Here we report ATP-responsive zeolitic imidazole framework-90 (ZIF-90) as a general platform for cytosolic protein delivery and CRISPR/Cas9 genome editing. The self-assembly of imidazole-2-carboxaldehyde and Zn2+ with protein forms ZIF-90/protein nanoparticles and efficiently encapsulates protein. It was found that, in the presence of ATP, the ZIF-90/protein nanoparticles are degraded to release protein due to the competitive coordination between ATP and the Zn2+ of ZIF-90. Intracellular delivery studies showed that the ZIF-90/protein nanoparticle can deliver a large variety of proteins into the cytosol, regardless of protein size and molecular weight. The delivery of cytotoxic RNase A efficiently prohibits tumor cell growth, while the effective delivery of genome-editing protein Cas9 knocks out the green fluorescent protein (GFP) expression of HeLa cells with efficiency up to 35%. Given the fact that ATP is upregulated in disease cells, it is envisaged that the ATP-responsive protein delivery will open up new opportunities for an advanced protein delivery and CRISPR/Cas9 genome editing for targeted disease treatment. ...Hide

DOI：10.1021/jacs.8b11996
百度学术：Nanoscale ATP-Responsive Zeolitic Imidazole Framework-90 as a General Platform for Cytosolic Protein Delivery and Genome Editing
语言：外文
被引频次：26
人气指数：2
浏览次数：2
基金：National Key Research and Development Program of China [2017YFA0208100, 2016YFA0200104]; National Science Foundation of ChinaNational Natural Science Foundation of China [21778056, 21790390, 21790391, 21621062, 21435007]
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