文献详情
Therapeutic hypothermia effectively reduces elevated extracellular ascorbate concentrations caused by acute spinal cord injury
文献类型：期刊
通讯作者：Zhou, F (reprint author), Peking Univ, Hosp 3, Dept Orthoped, 49 North Garden Rd, Beijing 100191, Peoples R China.
期刊名称：ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY影响因子和分区
年：2019
卷：47
期：1
页码：22-29
ISSN：2169-1401
关键词：Online electrochemical system; in vivo microdialysis; ascorbate; spinal cord injury
所属部门：化学系
摘要：In recent years, systemic hypothermia has taken the spotlight for its use in spinal cord injury (SCI) research fields, but detailed molecular mechanisms are still not fully understood. In this study, we use an online-electrochemical system (OECS) to in vivo continuously monitor the ascorbate of the rats' spinal cord. We find that the basal level of ascorbate in rat spinal cord is 1.85 +/- 0.88molL(-1) (n=20). It increased immediately after SCI and reached 2.36 +/- 0.65molL(-1) (164.90%+/- 7.99% ...More
In recent years, systemic hypothermia has taken the spotlight for its use in spinal cord injury (SCI) research fields, but detailed molecular mechanisms are still not fully understood. In this study, we use an online-electrochemical system (OECS) to in vivo continuously monitor the ascorbate of the rats' spinal cord. We find that the basal level of ascorbate in rat spinal cord is 1.85 +/- 0.88molL(-1) (n=20). It increased immediately after SCI and reached 2.36 +/- 0.65molL(-1) (164.90%+/- 7.99% of the basal level) (n=5) at 60min after the injury. The SCI-induced extracellular ascorbate increase is obviously attenuated by therapeutic hypothermia (28 degrees C) after injury and ascorbate returns to 3.01 +/- 0.59molL(-1) (100.24%+/- 5.02% of the basal level) (n=5), at 60min after SCI. These results substantially manifest that the OECS for ascorbate detection could be employed as a platform for understanding the pathological changes during spinal cord injury. This study provides experimental evidence for the essential roles of ascorbate in SCI which could serve as a biomarker for SCI. Our findings also raise the possibility that therapeutic hypothermia can effectively exert neuroprotection in the acute phase of SCI. ...Hide

DOI：10.1080/21691401.2018.1541136
百度学术：Therapeutic hypothermia effectively reduces elevated extracellular ascorbate concentrations caused by acute spinal cord injury
语言：外文
被引频次：1
基金：National Natural Science Foundation of ChinaNational Natural Science Foundation of China [51473004]
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