金文标2,
涂仁杰2,
郭艳梅2,
周旭2
1.深圳市罗湖区环境保护和水务局，深圳 518007
2.哈尔滨工业大学深圳，深圳微藻生物能源工程实验室，深圳 518055
基金项目: 深圳市科技计划资助项目（JCYJ20170307150223308，KJYY20171011144235970）
Development and operation of algal-bacterial symbiotic biological contact turntable reactor
ZHANG Jianqiao1,,JIN Wenbiao2,
TU Renjie2,
GUO Yanmei2,
ZHOU Xu2
1.Luohu District Environmental Protection and Water Affairs Bureau, Shenzhen 518007, China
2.Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology Shenzhen, Shenzhen 518055, China
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摘要:借助城市污水培养高脂微藻，既达到培养微藻目的，又可有效处理污水。将藻菌共生系统与生物接触转盘反应器有机结合，研制藻菌共生生物接触转盘反应器。在反应器内接种高脂二形栅藻（Scenedesmus dimorphus），与反应器内细菌构建菌藻共生系统，进入系统的城市污水为微藻生长补给氮、磷等元素，体系中的细菌分解有机物为微藻补给碳源。微藻光合作用产生的氧气，为细菌代谢提供保障。通过运行反应器小试装置，探究了反应器最优进水负荷、微藻的生长状况以及反应器对污水中目标污染物的去除效能。结果表明：该体系相对最优有机负荷（以COD计）为2.71 kg·（m3·d）-1，藻接种体积占反应器总体积的9.4%，用外加光源强化后，反应池中生物量明显提高1.50×106、1.75×106 和 2.10×106 cell·mL-1 ；此外，体系引入微藻后，氨氮去除率也得到显著促进。
关键词: 藻菌共生/
城市污水/
生物转盘/
二形栅藻
Abstract:The use of municipal sewage to cultivate high-fat microalgae can not only realize the harmless treatment of sewage, but also recover microalgae to prepare biodiesel. In this study, a biological contact turntable reactor and an algal-bacterial symbiotic system were combined to design an algal-bacterial symbiotic biological contact turntable reactor. The reactor was inoculated with Scenedesmus dimorphus, the sewage provided nitrogen and phosphorus for the growth of microalgae. In algal-bacterial symbiotic system, bacteria decomposed organic matter in the sewage and provided carbon source for microalgae, while the microalgae produced oxygen for the growth of bacteria through photosynthesis. A laboratory test equipment was used as the research object. The optimal influent load of the reactor, the growth status of the microalgae in the reactor and the removal of pollutants from the reactor were investigated. The results showed that the system has a relatively optimal organic load (measured as COD) of 2.71 kg·(m3·d)-1.The inoculation volume of Scenedesmus dimorphus accounted for 9.4% of the total volume of the reactor, and the final cell concentration in the three reaction cells was 1.50×106, 1.75×106 and 2.10×106 cell·mL-1 when external light source was added to the system. The addition of microalgae increased the ammonia nitrogen and nitrogen and phosphorus removal efficiency, but the promotion of organic matter removal was not obvious.
Key words:algal-bacterial symbiosis/
municipal wastewater/
biological turntable/
Scenedesmus dimorphus.
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| [2] | HAN S F, JIN W B, TU R J, et al.Biofuel production from microalgae as feedstock: Current status and potential [J].Critical Reviews in Biotechnology,2015,35(2):255-268 10.3109/07388551.2013.835301 |
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| [11] | 郭艳梅. 新型藻菌生物转盘反应器的研制[D]. 哈尔滨：哈尔滨工业大学,2010 |
| [12] | 张倩倩. 生物转盘处理城镇污水的中试试验研究[D]. 淮南：安徽理工大学,2014 |
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藻菌共生生物接触转盘反应器的研制和运行
张剑桥1,,金文标2,
涂仁杰2,
郭艳梅2,
周旭2
1.深圳市罗湖区环境保护和水务局，深圳 518007
2.哈尔滨工业大学深圳，深圳微藻生物能源工程实验室，深圳 518055
基金项目: 深圳市科技计划资助项目（JCYJ20170307150223308，KJYY20171011144235970）
关键词: 藻菌共生/
城市污水/
生物转盘/
二形栅藻
摘要:借助城市污水培养高脂微藻，既达到培养微藻目的，又可有效处理污水。将藻菌共生系统与生物接触转盘反应器有机结合，研制藻菌共生生物接触转盘反应器。在反应器内接种高脂二形栅藻（Scenedesmus dimorphus），与反应器内细菌构建菌藻共生系统，进入系统的城市污水为微藻生长补给氮、磷等元素，体系中的细菌分解有机物为微藻补给碳源。微藻光合作用产生的氧气，为细菌代谢提供保障。通过运行反应器小试装置，探究了反应器最优进水负荷、微藻的生长状况以及反应器对污水中目标污染物的去除效能。结果表明：该体系相对最优有机负荷（以COD计）为2.71 kg·（m3·d）-1，藻接种体积占反应器总体积的9.4%，用外加光源强化后，反应池中生物量明显提高1.50×106、1.75×106 和 2.10×106 cell·mL-1 ；此外，体系引入微藻后，氨氮去除率也得到显著促进。
English Abstract
Development and operation of algal-bacterial symbiotic biological contact turntable reactor
ZHANG Jianqiao1,,JIN Wenbiao2,
TU Renjie2,
GUO Yanmei2,
ZHOU Xu2
1.Luohu District Environmental Protection and Water Affairs Bureau, Shenzhen 518007, China
2.Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology Shenzhen, Shenzhen 518055, China
Keywords: algal-bacterial symbiosis/
municipal wastewater/
biological turntable/
Scenedesmus dimorphus
Abstract:The use of municipal sewage to cultivate high-fat microalgae can not only realize the harmless treatment of sewage, but also recover microalgae to prepare biodiesel. In this study, a biological contact turntable reactor and an algal-bacterial symbiotic system were combined to design an algal-bacterial symbiotic biological contact turntable reactor. The reactor was inoculated with Scenedesmus dimorphus, the sewage provided nitrogen and phosphorus for the growth of microalgae. In algal-bacterial symbiotic system, bacteria decomposed organic matter in the sewage and provided carbon source for microalgae, while the microalgae produced oxygen for the growth of bacteria through photosynthesis. A laboratory test equipment was used as the research object. The optimal influent load of the reactor, the growth status of the microalgae in the reactor and the removal of pollutants from the reactor were investigated. The results showed that the system has a relatively optimal organic load (measured as COD) of 2.71 kg·(m3·d)-1.The inoculation volume of Scenedesmus dimorphus accounted for 9.4% of the total volume of the reactor, and the final cell concentration in the three reaction cells was 1.50×106, 1.75×106 and 2.10×106 cell·mL-1 when external light source was added to the system. The addition of microalgae increased the ammonia nitrogen and nitrogen and phosphorus removal efficiency, but the promotion of organic matter removal was not obvious.
