中文关键词镉污染芒草高通量测序分子生态网络分析PICRUSt分析 英文关键词cadmium contaminationMiscanthushigh-throughput sequencingphylogenetic molecular ecological networksPICRUSt analysis

作者	单位	E-mail
陈兆进	南阳师范学院水资源与环境工程学院, 河南省南水北调中线水源区流域生态安全国际联合实验室, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061	zhaojin_chen@163.com
林立安	南阳师范学院生命科学与农业工程学院, 南阳 473061
李英军	南阳师范学院生命科学与农业工程学院, 南阳 473061
陈彦	南阳师范学院生命科学与农业工程学院, 南阳 473061
张浩	南阳师范学院生命科学与农业工程学院, 南阳 473061
韩辉	南阳师范学院生命科学与农业工程学院, 南阳 473061
吴乃成	德国基尔大学水文与水资源管理系, 基尔 24098
Nicola Fohrer	德国基尔大学水文与水资源管理系, 基尔 24098
李玉英	南阳师范学院水资源与环境工程学院, 河南省南水北调中线水源区流域生态安全国际联合实验室, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061
任学敏	南阳师范学院生命科学与农业工程学院, 南阳 473061	renxuemin2520@126.com

中文摘要 芒草（Miscanthus）作为第二代能源植物，已用于重金属污染土壤修复研究，但目前该过程中土壤细菌群落组成和功能研究开展较少.以芒草品种南荻（M.saccariflorus）为研究对象，通过高通量测序结合分子生态网络分析和PICRUSt功能预测，分析研究100 mg·kg^-1 Cd的胁迫对芒草根际细菌群落组成、共发生网络和功能的影响.MiSeq测序表明芒草根际细菌群落由32个门和425个属的细菌组成，包含鞘氨醇单胞菌属（Sphingomonas）、芽孢杆菌属（Bacillus）、芽单胞菌属（Gemmatimonas）和链霉菌属（Streptomyces）等植物促生细菌（PGPR）种群.相似性分析（ANOSIM）和非参数多元方差分析（Adonis）表明，Cd的添加能显著影响芒草根际细菌群落组成，降低其群落多样性.同时分子生态网络分析表明Cd的添加降低了芒草根际细菌之间的相互作用，导致其网络结构更为简单；降低了网络缓冲环境变化的能力；提高了负相关连线数，使根际细菌物种之间的竞争关系更强；改变关键细菌组成.PICRUSt功能预测分析表明Cd的胁迫降低了芒草根际土壤细菌功能.本研究初步分析了芒草根际细菌群落组成及其对镉胁迫的响应，为后续调控芒草修复效率提供了基础. 英文摘要 As a representative of second-generation bioenergy plants, Miscanthus has received increasing attention in the studies of heavy metal (HM)-contaminated soil remediation. Currently, few studies have examined the effects of using Miscanthus to remediate HM-contaminated soils on the composition and function of microbial communities. In this study, the Miscanthus cultivar M. saccariflorus was examined for its tolerance and enrichment abilities when grown in soils containing 100 mg ·kg^-1 of cadmium (Cd). The structure, function, and co-occurrence network of their rhizosphere bacterial communities were analyzed during the remediation process. MiSeq sequencing showed that the Miscanthus rhizosphere bacterial community comprised 32 phyla and 425 genera, including plant growth-promoting rhizobacteria (PGPR), such as Sphingomonas, Bacillus, Gemmatimonas, and Streptomyces. The addition of Cd affected the Miscanthus rhizosphere bacterial community and reduced community diversity. Phylogenetic molecular ecological networks indicated that Cd addition reduced the interactions between Miscanthus rhizosphere bacteria to generate a simpler network structure, increased the number of negative-correlation links, enhanced the competition between rhizosphere bacterial species, and changed the composition of key bacteria. PICRUSt functional predictive analysis indicated that Cd stress reduced soil bacterial functions in the Miscanthus rhizosphere. The results of this study provide a reference for the subsequent regulation of efficient Miscanthus remediation by PGPRs or key bacteria.

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