中文关键词
水稻镉(Cd)褪黑素2,4-表油菜素内酯茉莉酸抗氧化系统 | 英文关键词riceCdmelatonin2,4-epibrassinolidejasmonic acidantioxidant system |
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| 中文摘要 |
| 为了探讨外源添加植物激素对Cd胁迫下水稻幼苗的抗氧化系统及Cd吸收积累情况的影响，减少Cd在植物体内的运输和积累，从而来缓解Cd对水稻的胁迫.以中嘉早17水稻幼苗为研究对象，进行水培试验，设置0、5和25 μmol·L-1这3个Cd浓度处理，3种外源植物激素处理：不添加植物激素、100 μmol·L-1褪黑素（MT）、0.2 μmol·L-12，4-表油菜素内酯（EBL）和0.2 μmol·L-1茉莉酸（JA），共12个处理，每个处理重复3次.测定水稻幼苗体内Cd的含量，同时也对水稻幼苗地上部及根部丙二醛（MDA）、过氧化物酶（POD）、过氧化氢酶（CAT）和还原型谷胱甘肽（GSH）含量进行分析.结果表明，在5 μmol·L-1和25 μmol·L-1 Cd胁迫下，外源添加MT、EBL和JA使地上部MDA含量显著降低了11%~24%，但是根系与地上部情况恰好相反，添加3种外源物质均导致根系中MDA含量增加，其中MT和EBL现象明显，在5 μmol·L-1 Cd胁迫下，与CK相比，分别提高了45.5%和20.0%；在25 μmol·L-1 Cd胁迫下，分别提高了46.2%和19.8%.外源添加植物激素可显著增加水稻幼苗地上部和地下部POD、CAT的活性，降低GSH以及Cd的含量，在5 μmol·L-1 Cd胁迫下，添加MT、EBL和JA导致水稻地上部Cd含量分别降低39.4%、40.1%和51.6%，根部分别降低38.9%、40.2%和7.0%；25 μmol·L-1 Cd胁迫下，地上部Cd含量分别降低18.9%、14.5%和35.6%，根部分别降低85.3%、81.1%和56.5%.由此可见，通过外源添加低浓度植物激素MT、EBL和JA，可缓解Cd对水稻的胁迫，降低Cd对水稻的毒害作用. |
| 英文摘要 |
| In order to explore the effects of the exogenous addition of plant hormones on the antioxidant system and Cd absorption and accumulation of rice seedlings under Cd stress, the transportation and accumulation of Cd was reduced in plants to alleviate the stress of Cd on the rice. With the rice seedlings of Zhongjiazao 17 as the research object, a hydroponic experiment was carried out with three Cd concentration treatments (0, 5, and 25 μmol·L-1), and four exogenous plant hormone treatments:no plant hormones, 100 μmol·L-1 melatonin (MT), 0.2 μmol·L-1 2,4-epibrassinolide (EBL), and 0.2 μmol·L-1 jasmonic acid (JA), for a total of 12 treatments, each treatment repeated three times. The contents of Cd in the rice seedlings were analyzed, as well as the content of MDA, POD, CAT, and reduced GSH in the shoots and roots of the rice seedlings. The results indicated that under the stress of 5 μmol·L-1 and 25 μmol·L-1 Cd, the addition of MT, EBL, and JA significantly reduced the MDA content of the shoots by 11%-24%, and the roots and shoots were healthy. On the contrary, the addition of the three exogenous substances all caused an increase in the MDA content in the root system, but the effects of MT and EBL were obvious. Under the 5 μmol·L-1 Cd stress, compared with CK, the MDA contents increased by 45.5% and 20.0% respectively; under 25 μmol·L-1 Cd stress, they increased by 46.2% and 19.8%. The exogenous addition of plant hormones can significantly increase the activity of POD and CAT in the shoots and underground parts of the rice seedlings and reduce the contents of GSH and Cd. Under the 5 μmol·L-1 Cd stress, the Cd content in the shoots of rice plants decreased by 39.4%, 40.1%, and 51.6%, the roots were reduced by 38.9%, 40.2%, and 7.0%. Under the 25 μmol·L-1 Cd stress, the aboveground Cd content was reduced by 18.9%, 14.5%, and 35.6%, and the roots were reduced by 85.3%, 81.1%, and 56.5%. By exogenously adding low-concentration plant hormones MT, EBL, and JA, the stress of Cd on the rice can be alleviated, and the toxic effect of Cd on rice can be reduced. |
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