中文关键词丹江口水库浮游真菌群落组成高通量测序FUNGuild 英文关键词Danjiangkou Reservoirplanktonic fungicommunity structurehigh-throughput sequencingFUNGuild

作者	单位	E-mail
郑保海	南阳师范学院水资源与环境工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061	baohaizheng1981@163.com
王晓宇	南阳师范学院水资源与环境工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061
李英军	南阳师范学院水资源与环境工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061
陈彦	南阳师范学院水资源与环境工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061
李百炼	河南省南水北调中线水源区流域生态安全国际联合实验室, 南阳 473061 加州大学河滨分校环境科学系, 河滨 CA92521, 美国
李玉英	南阳师范学院水资源与环境工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061 河南省南水北调中线水源区流域生态安全国际联合实验室, 南阳 473061	lyying200508@163.com
陈兆进	南阳师范学院水资源与环境工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061 河南省南水北调中线水源区流域生态安全国际联合实验室, 南阳 473061	zhaojin_chen@163.com

中文摘要 浮游真菌是水生态系统的重要组成部分，研究其群落组成和功能对于管理和维护丹江口水库生态环境具有重要意义.本实验采用Illumina公司MiSeq测序平台对2019年10月丹江口水库表层浮游真菌群落组成进行分析，并结合FUNGuild功能预测分析其生态功能.MiSeq测序结果表明丹江口水库浮游真菌由6门和213属组成，其中子囊菌门（Ascomycota）和担子菌门（Basidiomycota）为优势种群.丹江口水库水质监测结果表明，其水质整体处于良好状态，符合Ⅰ或Ⅱ饮用水源标准.RDA分析表明氮磷营养盐（TN、TP）、水温（T）、氧化还原电位（ORP）和卡尔森营养状态指数（TLI）是影响浮游真菌分布的重要因素.Spearman秩相关分析表明，理化因子主要与链格孢属（Alternaria）、芽枝霉属（Cladosporium）、青霉属（Penicillium）、路德酵母属（Lodderomyces）和Acremonium等优势属呈显著相关.FUNGuild功能预测分析表明丹江口浮游真菌的营养型和功能群主要为病理营养型（pathotroph）、腐生营养型（saprotroph）和病理-腐生营养型（pathotroph-saprotroph），其中黑鸡嘴样品植物病原菌（plant pathogen）和动物病原菌（animal pathogen）比例显著高于其他样品.本研究初步分析了丹江口库区浮游真菌群落组成和生态功能，表明其具有一定程度的潜在生态风险，后续丹江口水库生物监测中浮游真菌监测需要加以重视. 英文摘要 Planktonic fungi are important components of aquatic ecosystems, and analyses of their community composition and function have far-reaching significance for the ecological management and maintenance of the Danjiangkou reservoir. The composition and function of the planktonic fungal community in the surface water layer of the Danjiangkou Reservoir in October 2019 was investigated using Illumina MiSeq sequencing combined with FUNGuild analyses. According to the results, the reservoir community is primarily composed of 6 phyla 213 genera, with Ascomycota and Basidiomycota being the dominant phyla. The water quality monitoring results for the Danjiangkou Reservoir met the Grade Ⅰ or Ⅱ water quality standards for the Environmental Quality Standards for Surface Water (GB 38382-2002). A redundancy analysis (RDA) of the planktonic fungal community and environmental factors showed that TN, TP, T, ORP, and TLI are important factors influencing the distribution of planktonic fungi. The Spearman correlation analysis showed that Alternaria, Cladosporium, Penicillium, Lodderomyces, and Acremonium were significantly correlated with physical and chemical water quality parameters. FUNGuild was used to predict the nutritional and functional groups of planktonic fungi, and the results showed that pathotrophs, saprotrophs, and pathotroph-saprotrophs were the major components. The pathotroph composition analysis showed that the proportions of plant pathogens and animal pathogens in the Heijizui samples were significantly higher than those observed in the other monitoring sites. The community composition, function, and influencing factors of the planktonic fungi community in the Danjiangkou Reservoir were investigated and indicated that it is potentially at ecological risk and more attention needs to be paid to planktonic fungi in the biological monitoring of water quality.

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