中文关键词汉江流域沉积物硝化速率amoA基因人为干扰理化因子 英文关键词Hanjiang River Basinsedimentpotential nitrification rateamoA genehuman disturbanceenvironment factors

作者	单位	E-mail
赵彬洁	中国科学院武汉植物园水生植物与流域生态重点实验室, 武汉 430074 中国科学院大学, 北京 100049	zhaobinjie@wbgcas.cn
王旭	中国科学院武汉植物园水生植物与流域生态重点实验室, 武汉 430074 西藏大学青藏高原生态与环境研究中心, 拉萨 850000 西藏大学理学院, 拉萨 850000
张健	中国科学院武汉植物园水生植物与流域生态重点实验室, 武汉 430074 西藏大学青藏高原生态与环境研究中心, 拉萨 850000 西藏大学理学院, 拉萨 850000
谭香	中国科学院武汉植物园水生植物与流域生态重点实验室, 武汉 430074
何睿	中国科学院武汉植物园水生植物与流域生态重点实验室, 武汉 430074 中国科学院大学, 北京 100049
周全	中国科学院武汉植物园水生植物与流域生态重点实验室, 武汉 430074 中国科学院大学, 北京 100049
史航	中国科学院武汉植物园水生植物与流域生态重点实验室, 武汉 430074 中国科学院大学, 北京 100049
张全发	中国科学院武汉植物园水生植物与流域生态重点实验室, 武汉 430074	qzhang@wbgcas.cn

中文摘要 河流是地表连接陆地和海洋生态系统生源要素的关键通道，河流生态系统中包括硝化作用在内的氮转化过程对全球氮循环具有重要影响.本研究选择位于秦岭南坡流域内人为干扰强度低的金水河及人为干扰强度高的淇河进行比较研究，探讨人为干扰强度对河流潜在硝化速率及硝化功能基因丰度的影响.结果表明，同时期的淇河潜在硝化速率显著高于金水河（P<0.05）.金水河和淇河的AOA-amoA基因丰度都显著高于AOB-amoA（P<0.05），但枯水期淇河的AOB-amoA基因丰度显著高于金水河，金水河与淇河AOA-amoA基因丰度无显著差异.潜在硝化速率与水温、pH、水体NO₃^-和沉积物NO₃^-浓度呈显著正相关（P<0.05）.硝化功能基因丰度与水温、pH、水体及沉积物NO₃^-、NH₄⁺及有机碳浓度相关性显著（P<0.05）.潜在硝化速率与AOA-amoA基因丰度无显著相关性，但与AOB-amoA基因丰度呈负相关.本研究表明人为干扰强度高的河流具有更高的潜在硝化速率及硝化功能基因丰度，潜在硝化速率及硝化功能基因丰度与河流水体及沉积物的理化因子显著相关.本研究对于河流生态系统氮素迁移转化过程有重要意义. 英文摘要 In the context of increasing nitrogen loading in river systems worldwide, it is critically important to understand nitrification to maintain river ecosystem health. This comparative study was conducted to explore the relationships among the intensity of human disturbance, environmental factors, potential nitrification rate, and functional gene abundance in the Jinshui River (slightly disturbed basin) and the Qihe River (intensely disturbed basin). The results showed that AOA-amoA gene abundance was higher than that of AOB-amoA. The potential nitrification rate in the Qihe River was higher than that in the Jinshui River. There was no significant difference in AOA-amoA gene abundance between the two rivers; however, the AOB-amoA gene abundance in the Qihe River was significantly higher than that in the Jinshui River in the low flow season. The abundance of nitrification functional genes was significantly correlated with water temperature, pH, NO₃^-, NH₄⁺, and organic carbon. The potential nitrification rate was positively correlated with water temperature, pH, and NO₃^-, and negatively correlated with AOB-amoA gene abundance. These results showed that the intensely disturbed sites had higher potential nitrification rates and nitrification functional genes abundances, and potential nitrification rates and abundances of nitrification function genes were significantly related to physical and chemical water and sediment factors. This study will be important for nitrogen pollution control in the river systems.

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