中文关键词厌氧氨氧化(ANAMMOX)前置反硝化中晚期垃圾渗滤液微生物多样性定量PCR 英文关键词anaerobic ammonia oxidation(ANAMMOX)pre-denitrificationmature landfill leachatemicrobial diversityQ-PCR

作者	单位	E-mail
陆明羽	苏州科技大学环境科学与工程学院, 苏州 215009 苏州科技大学环境生物技术研究所, 苏州 215009	lu_mingyu@qq.com
李祥	苏州科技大学环境科学与工程学院, 苏州 215009 苏州科技大学环境生物技术研究所, 苏州 215009
黄勇	苏州科技大学环境科学与工程学院, 苏州 215009 苏州科技大学环境生物技术研究所, 苏州 215009	yhuang@mail.usts.edu.cn
殷记强	苏州科技大学环境科学与工程学院, 苏州 215009 苏州科技大学环境生物技术研究所, 苏州 215009
方文烨	苏州科技大学环境科学与工程学院, 苏州 215009 苏州科技大学环境生物技术研究所, 苏州 215009

中文摘要 为了推进厌氧氨氧化（anaerobic ammonia oxidation，ANAMMOX）脱氮工艺在垃圾渗滤液处理方面的应用，在某垃圾填埋场建立了不同反硝化（denitrification，DN）与短程硝化-厌氧氨氧化（partial nitrification-ANAMMOX，PN-ANAMMOX）耦合模式的中试反应器处理垃圾渗滤液，探讨其耦合模式对脱氮及微生物群落结构的影响.结果表明DN+（PN-ANAMMOX）工艺可以将DN耦合入PN-ANAMMOX进行脱氮，但随着渗滤液中有机物浓度的增加，DN+（PN-ANAMMOX）工艺的PN区的需氧量增加，Nitrosomonadaceae科菌的富集受到限制.而NO₂^--N的供给不足进一步导致ANAMMOX区Brocadiaceae科微生物的富集也受到限制，总氮去除速率（total nitrogen removal rate，TNRR）停留在0.44 kg ·（m³ ·d）^-1.而在DN-（PN-ANAMMOX）工艺中，具有反硝化能力的Saprospiraceae科菌在DN区富集，有机物主要在DN区被降解去除，为后续PN-ANAMMOX提供了良好的低碳环境.Nitrosomonadaceae科及Brocadiaceae科菌在相应的PN区及ANAMMOX区得到富集，反应器的TNRR和总氮去除率（total nitrogen removal efficiency，TNRE）也进一步提升至0.55 kg ·（m³ ·d）^-1和94.65%，实现了对NH₄⁺-N和有机物浓度分别为2233 mg ·L^-1和2712 mg ·L^-1渗滤液的直接处理.其中Candidatus Kuenenia菌更能适应高基质浓度的渗滤液水质，成为ANAMMOX区的优势菌属. 英文摘要 To promote the application of ANAMMOX process in landfill leachate treatment, a pilot reactor based on the ANAMMOX process was established at a landfill site. In this paper, we aim to further analyze the influence of different coupling modes of denitrification (DN) and partial nitrification and ANAMMOX (PN-ANAMMOX) on the diversity of microbial community. The DN+(PN-ANAMMOX) process could effectively treat the mature leachate. However, with an increase in organic matter in the influent, the oxygen demand of PN zone increased, and the enrichment of Nitrosomonadaceae in the PN zone was limited. The lack of substrate supply for ANAMMOX zone further limited the enrichment of Brocadiaceae as well; thus, the total nitrogen removal rate (TNRR) remained at 0.44 kg ·(m³ ·d)^-1. In the DN-(PN-ANAMMOX) process, Saprospiraceae with denitrifying ability was enriched in the DN zone, and the organic matter was gradually degraded and removed; thus, a good low-carbon environment was provided for the subsequent PN-ANAMMOX process. Nitrosomonadaceae and Brocadiaceae were enriched in the functional zones, and the TNRR and total nitrogen removal efficiency (TNRE) of the DN-(PN-ANAMMOX) were further elevated to 0.55 kg ·(m³ ·d)^-1 and 94.65%, respectively. Moreover, the direct treatment of mature leachate with 2233 mg ·L^-1 NH₄⁺-N and 2712 mg ·L^-1 COD was finally realized. In addition, Candidatus Kuenenia was better adapted to leachate and high substrate concentration wastewater, and it became the dominant genus in the ANAMMOX zone.

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