中文关键词ABR-MBR耦合工艺反硝化除磷无污泥回流微生物群落高通量测序 英文关键词ABR-MBR coupling processdenitrifying phosphorus removalno sludge refluxmicrobial communityhigh throughput sequencing

作者	单位	E-mail
缪新年	苏州科技大学环境科学与工程学院, 苏州 215009	18262036697@163.com
汪倩	苏州科技大学环境科学与工程学院, 苏州 215009
郭凯成	苏州科技大学环境科学与工程学院, 苏州 215009
刘文如	苏州科技大学环境科学与工程学院, 苏州 215009 江苏省水处理技术与材料协同创新中心, 苏州 215009 城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
沈耀良	苏州科技大学环境科学与工程学院, 苏州 215009 江苏省水处理技术与材料协同创新中心, 苏州 215009 城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009 江苏省环境科学与工程重点实验室, 苏州 215009	ylshen@mail.usts.edu.cn

中文摘要 探究ABR-MBR耦合工艺在无污泥回流及在ABR各隔室接种高浓度（25g ·L^-1，以MLSS计）活性污泥条件下启动反硝化除磷性能的可行性以及反硝化除磷隔室内微生物群落特征.结果表明，通过逐步提升硝化液回流比（R）由0%至200%成功启动反硝化除磷性能.稳定运行过程中，系统对COD、PO₄^3--P和TN的平均去除率分别为88.28%、54.45%和61.93%.在ABR进水容积负荷（以COD计，下同）为0.8 kg ·（m³ ·d）^-1、R为150%、ABR和MBR的水力停留时间（HRT）分别为9h和3.3 h时，VFA平均产量为80.58mg ·L^-1、回流硝化液的ρ（NO₂^--N）/ρ（NO₃^--N）平均为1.68，PO₄^3--P和TN的平均去除率分别为64.94%和62.95%，实现短程硝化和反硝化除磷耦合.批次实验证明反硝化除磷菌（DPAOs）为ABR中主要除磷功能菌，厌氧释磷量和缺氧吸磷量分别为3.73mg ·L^-1和10.22mg ·L^-1.高通量测序分析结果表明，除磷隔室内微生物的优势菌门为变形菌门（Proteobacteria）和拟杆菌门（Bacteroidetes），分别占23.49%~53.66%和16.55%~21.78%，与反硝化除磷有关的功能微生物主要是变形菌门下的索氏菌属（Thauera）、发硫菌属（Thiothrix）、假单胞菌属（Pseudomonas）、norank_f_Rhodocyclaceae和unclassified_f_Rhodocyclaceae，拟杆菌门中的鞘脂杆菌目（Sphingobacteriales）. 英文摘要 The feasibility of the denitrifying phosphorus removal process in the ABR-MBR system with no sludge reflux and high concentration of seeding activated sludge (25 g ·L^-1, in MLSS) in the ABR was investigated. The characteristics of the microbial community in the denitrifying phosphorus removal compartment were also evaluated. The denitrifying phosphorus removal function was achieved by gradually increasing the reflux ratio (R) from 0% to 200%. During the stable operation, the average removal rates of COD, PO₄^3--P, and TN in the system were 88.28%, 54.45%, and 61.93%, respectively. When the influent loading rate, NO_x^--N reflux ratio, and hydraulic retention time (HRT) of ABR and MBR were 0.8 kg ·(m³ ·d)^-1, 150%, and 9 h and 3.3 h, respectively, the average VFA concentration of 80.58 mg ·L^-1, ρ(NO₂^--N)/ρ(NO₃^--N) reflux ratio of 1.68, and PO₄^3--P and TN removal rates of 64.94% and 62.95% were obtained. The short-cut nitrification denitrifying phosphorus removal was achieved in the ABR-MBR system. Batch tests showed that denitrifying phosphorus removal bacteria (DPAOs) were the main functional bacteria in the ABR, with anaerobic phosphorus release and anoxic phosphorus uptake of 3.73 mg ·L^-1 and 10.22 mg ·L^-1, respectively. High throughput sequencing results showed that Proteobacteria and Bacteroidetes were the dominant phyla in the phosphorus removal compartment, accounting for 23.49%-53.66% and 16.55%-21.78% of the total phyla, respectively. Thauera, Thiothrix, Pseudomonas, norank_ f_Rhodocyclaceae, and unclassification_ f_Rhodocyclaceae in Proteobacteria, and Sphingobacteriales in Bacteroidetes were the potential denitrifying phosphorus removal microorganisms.

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