中文关键词颗粒污泥全自养脱氮低温低基质DO/TANNOB抑制 英文关键词granular sludgecompletely autotrophic nitrogen removallow temperaturelow-strengthDO/TANNOB repressure

作者	单位	E-mail
刘雨馨	苏州科技大学环境科学与工程学院, 苏州 215009	perihelionliu@163.com
王建芳	苏州科技大学环境科学与工程学院, 苏州 215009 苏州科技大学天平学院, 苏州 215009 城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009	wjf302@163.com
钱飞跃	苏州科技大学环境科学与工程学院, 苏州 215009 城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009 江苏高校水处理技术与材料协同创新中心, 苏州 215009
陈倩苗	苏州科技大学天平学院, 苏州 215009
乔伟	苏州科技大学天平学院, 苏州 215009
沈聪	苏州科技大学天平学院, 苏州 215009

中文摘要 以室温培养的单级PN-ANAMMOX（PN/A）颗粒污泥为对象，基于颗粒污泥的全自养脱氮工艺，研究在低温条件下处理低浓度氨氮废水的脱氮效能及微生物群落结构.结果表明，在（15±1）℃条件下，维持氨氮负荷在1.29 kg ·（m³ ·d）^-1，进水氨氮质量浓度从70 mg ·L^-1逐级降低至40 mg ·L^-1，溶解氧比剩余氨氮（DO/TAN）维持在0.22~0.25，总氮去除率可维持在（85±4）%，出水总氮平均质量浓度约为8.9 mg ·L^-1，运行期内无亚硝酸氧化菌（nitrite-oxidizing bacteria，NOB）显著增殖，Nitrospira丰度小于1%.淘洗絮体污泥和控制低DO/TAN值可作为抑制NOB增殖的有效调控策略.全自养脱氮颗粒污泥在低温低基质条件下运行，颗粒粒径会变小，颜色由棕红色变为棕黄色.PS总量略有下降，PN/PS的比值稳定在2.5~3.浮霉菌门（Planctomycetes）和变形菌门（Proteobacteria）在微生物系统中占主导，污泥中存在Candidatus_Kuenenia和Candidatus_Brocadia两种厌氧氨氧化菌属. 英文摘要 A single-stage PN-ANAMMOX (PN/A) granular sludge cultured at room temperature was used to investigate the completely autotrophic nitrogen removal efficiency and microbial community structure of low-strength wastewater based on the completely autotrophic nitrogen removal over nitrite in granular sludge at a low temperature. The results showed that at the low temperature (15±1)℃, the ammonia nitrogen load was maintained at 1.29 kg ·(m³ ·d)^-1, and the ammonia nitrogen concentration in the injection was gradually reduced from 70 mg ·L^-1 to 40 mg ·L^-1. DO/TAN was controlled at 0.22-0.25. The total nitrogen removal rate was maintained at (85±4)%, and the average TN concentration in the effluent was 8.9 mg ·L^-1. There was no significant proliferation of nitrite-oxidizing bacteria (NOB) during the operation period, and the Nitrospira abundance was less than 1%. Elutriation of the floc sludge and the control of low DO/TAN values can be used as effective control strategies to inhibit NOB proliferation. Through completely autotrophic nitrogen removal over nitrite in granular sludge operated under low-temperature and low-substrate conditions, the particle size became smaller, and the color changed from brown red to brown yellow. The total amount of PS decreased slightly, and the ratio of PN/PS stabilized at 2.5-3.0. Planctomycetes and Proteobacteria dominated the community, and Candidatus_Kuenenia and Candidatus_Brocadia were two AMX bacteria in the sludge.

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