中文关键词温度硝化细菌亚硝酸盐氧化细菌比亚硝态氮氧化速率16S rRNA高通量测序 英文关键词temperaturenitrobacterianitrite-oxidizing bacteriaspecific nitrite oxidation rate16S rRNA high-throughput sequencing

作者	单位	E-mail
侯晓薇	兰州交通大学环境与市政工程学院, 兰州 730070	374463768@qq.com
牛永健	甘肃省轻工研究院, 兰州 730030
李维维	甘肃省轻工研究院, 兰州 730030
王光杰	山东同济测试科技股份有限公司, 烟台 264000
孙洪伟	兰州交通大学环境与市政工程学院, 兰州 730070 烟台大学环境与材料工程学院, 烟台 264005	12821306@qq.com

中文摘要 为了进一步了解在亚硝酸盐氧化过程中，高温冲击对活性污泥微生物菌群结构的影响，本研究中，以在不同NO₂^--N浓度条件下富集的硝化活性污泥为研究对象，利用16S rRNA高通量测序技术分析方法，通过改变环境温度考察活性污泥微生物菌群丰度变化及结构特征.高通量测序分析结果表明：25℃时易于微生物生长，系统活性污泥微生物菌群多样性最丰富.随着高温冲击试验进行，系统内菌群丰富度、均匀度和多样性呈下降趋势.此外，分析发现本系统主要硝化功能菌为Nitrospirae的Nitrospira，更适宜在35℃生长.且高温冲击试验同样引起了活性污泥中非硝化功能微生物（Bacteroidetes、Chloroflexi、Halomonas和Pseudomonas等）的菌群结构差异.试验结果可为高温冲击条件下亚硝酸盐氧化过程中微生物菌群分布特点的研究提供部分理论参考，并为相关高温冲击试验给予部分借鉴. 英文摘要 In order to further understand the influence of high temperature shock on the microbial community structure of activated sludge during the process of nitrite oxidation, the enriched nitrifying activated sludge under different NO₂^--N concentration was taken as the research object in this study. 16S rRNA high-throughput sequencing technology was used to analyze the changes in the microbial community abundance and structural characteristics of activated sludge by changing the environmental temperature. The results of high-throughput sequencing showed that microorganisms were more likely to grow at 25℃, and the diversity of the microbial community in the activated sludge was the most abundant. With increased temperature, the richness, evenness, and diversity of the flora in the system decreased. In addition, it was found that the main nitrifying bacterium in the system was Nitrospira of Nitrospirae, whereby 35℃ was more suitable for its growth. Meanwhile, a higher temperature also caused differences in the structure of non-nitrifying functional microorganisms (e.g., Bacteroidetes, Chlorofulexi, Halomonas, and Pseudomonas) in the activated sludge. The results of this study provide some theoretical reference for the investigation of the distribution characteristics of microbial flora during the process of nitrite oxidation under high temperature shock, and can also be used as reference for relevant high temperature shock tests.

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