中文关键词低溶解氧黑臭水体脱氮菌剂氮代谢底物浓度 英文关键词low dissolved oxygenblack odorous water bodydenitrifying agentnitrogen metabolismsubstrate concentration

作者	单位	E-mail
赵志瑞	河北地质大学水资源与环境学院, 石家庄 050031 中国科学院生态环境研究中心, 北京 100085 河北省水资源可持续利用与开发重点实验室, 石家庄 050031 河北省高校生态环境地质应用技术研发中心, 石家庄 050031	zhiruizh@163.com
刘硕	河北地质大学水资源与环境学院, 石家庄 050031
李铎	河北地质大学水资源与环境学院, 石家庄 050031
李海萍	河北科技大学理学院, 石家庄 050018
李方红	河北地质大学水资源与环境学院, 石家庄 050031
张佳瑶	中国科学院生态环境研究中心, 北京 100085
白志辉	中国科学院生态环境研究中心, 北京 100085	zhbai@rcees.ac.cn

中文摘要 研究在低溶解氧浓度下氨化细菌、硝化细菌、反硝化细菌氮代谢能力，明确不同菌剂脱氮效率和氮转化关系，为大湾区黑臭水体治理提供理论依据.通过不同菌剂浓度和不同底物浓度，检测筛选出的氨化细菌（Staphylococcus sp.Ay）、硝化细菌（Microbacterium sp.Xw）和反硝化细菌（Arthrobacter sp.Fy）制成的菌剂在污水处理厂排放的水中的氮代谢特征；然后在低溶解氧条件下，研究不同Ay、Xw和Fy菌剂浓度在同浓度黑臭水体中氮代谢变化情况、以及相同菌剂浓度在不同浓度黑臭水体中的氮代谢特征.结果表明：Ay菌剂细菌浓度增加3倍，氨化效率没有成倍增加，说明增加氨化细菌浓度不是增加氨化速率的最佳方法；Xw菌剂对硝态氮浓度变化影响显著，低菌剂浓度条件下60 h硝态氮浓度增加180%，高菌剂浓度为231%，Fy反硝化效率较高，在较低浓度下能很快去除硝态氮.总氮浓度的适当提高可以增加Ay菌剂的氨化效率，过高会抑制Ay菌剂的氮氮代谢效率.Xw菌剂硝态氮的生成率在总氮浓度25 mg·L^-1条件下达到最好效果，浓度提高3736%；Fy菌剂对氨氮去除效果不明显.总氮浓度为25 mg·L^-1时，Fy菌剂对硝态氮的去除达到最好效果.得出：氨化细菌和反硝化细菌原菌剂直接投加，氮代谢速率较高，硝化细菌菌剂浓度增加，可以提高硝态氮生成速率.在一定范围内，增加底物浓度可以增加菌剂氮代谢效率，浓度过高将抑制氮代谢效果，可为大湾区黑臭水体问题的解决提供数据支持. 英文摘要 In order to deal with low dissolved oxygen black odor water body of Guangdong-Hong Kong-Macao Greater Bay Area, the nitrogen metabolic capacity of ammoniated bacteria, nitrifying bacteria and denitrifying bacteria under low dissolved oxygen concentration was studied, and the relationship between denitrification efficiency and nitrogen conversion of different bacteria was clarified, which provided theoretical basis for the treatment of black odor water of Guangdong-Hong Kong-Macao Greater Bay Area. Nitrogen conversion characteristics of ammoniated bacteria (Staphylococcus sp. Ay), nitrifying bacteria (Microbacterium sp. Xw), and denitrifying bacteria (Arthrobacter sp. Fy) in simulated wastewater were detected by different concentration of bacteria and different concentration of substrates. The nitrogen metabolism of Ay, Xw and Fy in black odor water at the same concentration under low dissolved oxygen condition and the characteristics of nitrogen metabolism in different concentrations of black odor water were studied. The results showed that the bacterial concentration of Ay bacterium agent increased three times, but the ammoniation efficiency did not multiply. It indicated that increasing the concentration of ammoniated bacterium was not the best way to increase the ammoniation rate. Xw bacteria have a significant effect on the change of nitrate concentration. At 60 h, the nitrate concentration was increased 180% at low bacterial concentration, was 231% at a high bacterial concentration. Fy denitrification efficiency was high, that nitrate nitrogen can be removed quickly at lower concentration. Appropriate increase of total nitrogen concentration can increase the ammoniation efficiency of Ay microbial agent, but too high would inhibit the nitrogen metabolism efficiency of Ay microbial agent. The concentration of nitrate nitrogen of Xw bacterium reagent was increased (3736%) when the total nitrogen was 25 mg·L^-1. The Fy microbial agent has no obvious effect on ammonia nitrogen removal. When the total nitrogen concentration was 25 mg·L^-1, the removal rate of nitrate by Fy bacterium was the best. Overall, the results showed that the concentration of ammoniated bacteria and denitrifying bacteria could meet the need of nitrogen metabolism, and the nitrifying bacteria could increase the nitrate nitrogen synthesis rate with the increase of the concentration of nitrifying bacteria. In a certain range, increasing the concentration of substrate could increase the efficiency of nitrogen metabolism of bacterial agents, and excessive concentration would inhibit the effect of nitrogen metabolism. The study provides data support for the problem of black odor water in Guangdong-Hong Kong-Macao Greater Bay Area.

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