中文关键词细菌群落结构生物多样性活性污泥高通量测序淀粉废水 英文关键词bacterial community structurebiodiversityactivated sludgehigh-throughput sequencingwaste water containing starch

作者	单位	E-mail
赵婷婷	北京师范大学环境学院, 北京 100875	954962316@qq.com
乔凯	北京师范大学环境学院, 北京 100875
王蕾	北京师范大学环境学院, 北京 100875
张伟	北京师范大学环境学院, 北京 100875
竺建荣	北京师范大学环境学院, 北京 100875	zjrtshua@hotmail.com
唐一	南方科技大学工程技术创新中心, 北京 100083 北京南科大蓝色科技有限公司, 北京 100083
胡滨	南方科技大学工程技术创新中心, 北京 100083
王香莲	南方科技大学工程技术创新中心, 北京 100083 北京南科大蓝色科技有限公司, 北京 100083
杨静	山东西王糖业有限公司, 邹平 256209
景在涛	山东西王糖业有限公司, 邹平 256209

中文摘要 为探究淀粉废水处理活性污泥中微生物的群落结构及多样性，基于Illumina MiSeq高通量测序方法，分析了不同运行阶段的A/O处理系统中活性污泥的微生物群落与多样性组成.结果表明，A/O系统中处理淀粉生产废水的活性污泥在同一种废水下微生物群落结构总体比较稳定，优势细菌主要为变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）、绿弯菌门（Chloroflexi）、厚壁菌门（Firmicutes）和放线菌门（Actinobacteria）等；最重要优势细菌类群为变形菌门（45.66%~66.30%），其中γ-亚纲细菌是其主要成员，占比36.38%~66.65%.优势拟杆菌门主要成员鞘脂杆菌纲在污泥沉降性能较好时其占比下降，但绿弯菌门主要成员厌氧绳菌纲在污泥沉降性能较好时其占比明显增加，变化趋势正好相反，或许它们二者之间的耦合变化与污泥沉降性能变化密切相关.活性污泥样品中存在大量特殊功能菌群，它们在活性污泥的污染物分解和氮磷去除中发挥重要作用. 英文摘要 This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment. The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria. The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%. The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better. These changes may have been closely related to the behavior of sludge settleability. There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.

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