中文关键词抗生素抗性基因(ARGs)实时荧光PCR废水处理厂(WWTPs)工业园区芬顿氧化 英文关键词antibiotic resistance genes(ARGs)real-time PCRwastewater treatment plants(WWTPs)industrial parkFenton oxidation

作者	单位	E-mail
姚鹏城	上海师范大学环境与地理科学学院, 上海 200234 清华大学环境学院, 北京 100084	yaopc7283@163.com
陈嘉瑜	上海师范大学环境与地理科学学院, 上海 200234 清华大学环境学院, 北京 100084
张永明	上海师范大学环境与地理科学学院, 上海 200234
温东辉	北京大学环境科学与工程学院, 北京 100871
陈吕军	上海师范大学环境与地理科学学院, 上海 200234 清华大学环境学院, 北京 100084	chenlj@tsinghua.edu.cn

中文摘要 废水处理厂被认为是抗生素抗性基因（ARGs）的重要污染源.为探究抗性基因在进水状况复杂的废水处理厂沿程的分布变化特征，选取以生产抗生素为主导行业的某化工园区废水处理厂，使用实时荧光定量PCR对废水处理厂沿程ARGs的种类、丰度变化进行研究.结果表明，废水处理厂水体中检出16种ARGs，四环素类、磺胺类ARGs为废水处理厂中占主导的抗性基因，并检出可移动遗传元件intI 1，其丰度与磺胺类抗性基因的丰度（P<0.05，r>0.95）存在相关性，表明可移动遗传元件intI 1可能促进了磺胺类抗性基因的迁移和转化.园区医药企业以合成大环内酯类抗生素为主，由于选择性压力，园区废水中，ermB抗性基因的绝对丰度远远高于其他废水中ermB的绝对丰度.废水经过废水处理厂生物处理工艺，总ARGs绝对丰度下降了1.16个数量级，经过芬顿工艺处理后，总ARGs绝对丰度下降了2.46个数量级，表明该废水处理工艺中深度处理工艺对ARGs的去除效果优于生物处理.高浓度、可移动的ARGs已经存在于水体中，如果没有得到有效治理，从废水处理厂排出，将给环境带来高度风险. 英文摘要 Wastewater treatment plants (WWTPs) are regarded as the main source for antibiotic resistant genes (ARGs). To explore the features regarding the distribution of ARGs in wastewater with complicated composition in treatment plants, wastewater samples from a chemical industry park that produced antibiotics were selected. qPCR was applied to detect the type and abundance of ARGs in the wastewater flows from the WWTPs. The results indicated that 16 types of ARGs were detected from the wastewater from the WWTPs, among which sulfonamide resistance genes and tetracycline resistance genes were the dominant ARGs that appeared in the wastewater. Additionally, intI 1 was detected and its abundance was correlated with that of sulfonamide resistance genes. This indicated that intI 1 may promote the migration and transformation of sulfonamide resistance genes. The pharmaceutical factories in the park mainly synthesize macrolide antibiotics. Because of the selective pressure, the absolute abundance of ermB in the wastewater was much higher than that in the other industrial wastewater. The total ARGs decreased by 1.16 log via traditional biological treatment process, and the total ARGs decreased by 2.46 log via the Fenton process. The results showed that the removal effects of deep treatment processes on ARGs were better than that of biological treatment in this wastewater treatment process. Highly abundant and movable ARGs already exist in the water body, and their release from WWTPs without effective treatment poses high risks to the environment.

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