中文关键词厌氧氨氧化(ANAMMOX)颗粒污泥伴生菌盐度高通量测序 英文关键词anaerobic ammonium oxidation (ANAMMOX)granular sludgeassociated bacteriasalinityhigh-throughput sequencing

作者	单位	E-mail
王晗	重庆大学城市建设与环境工程学院, 重庆 400045	wanghan2202@163.com
李瀚翔	重庆大学城市建设与环境工程学院, 重庆 400045
陈猷鹏	重庆大学城市建设与环境工程学院, 重庆 400045
郭劲松	重庆大学城市建设与环境工程学院, 重庆 400045
晏鹏	重庆大学城市建设与环境工程学院, 重庆 400045
方芳	重庆大学城市建设与环境工程学院, 重庆 400045	fangfangcq@cqu.edu.cn

中文摘要 采用高通量测序技术探究了0、15和30 g·L^-1盐度条件下稳定运行ANAMMOX-EGSB反应器中颗粒污泥的微生物群落变化.结果发现，进水盐度提升至15 g·L^-1及30 g·L^-1后，反应器脱氮性能呈现小幅下降，随运行时间延长脱氮性能均可恢复.反应器性能稳定后，3种盐度条件下厌氧氨氧化菌的丰度依次为10.33%、20.90%和35.87%，其中Candidatus Kuenenia属为优势属.浮霉状菌门、变形菌门、绿弯菌门丰度占总体比例较高且累计丰度超过了80%，为反应器的优势菌门.盐度条件下，浮霉状菌门丰度增加，变形菌门丰度降低，绿弯菌门丰度相对稳定.电镜扫描显示盐度条件下颗粒污泥表面有大量丝状菌和胞外聚合物.盐度条件下反硝化菌丰度提高，增强了反硝化协同脱氮，绿弯菌门和拟杆菌门微生物丰度的提高有利于维持颗粒污泥结构稳定，好氧微生物及反硝化菌的存在也有利于维持反应器内部厌氧水平.这些结果表明，厌氧氨氧化菌经驯化可适应盐度，盐度条件下伴生菌对厌氧氨氧化菌功能的发挥提供了支撑. 英文摘要 High-throughput sequencing technology was used to investigate changes in the microbial community in granular sludge under salinity conditions of 0, 15, and 30 g·L^-1 in an ANAMMOX-EGSB reactor under stable operation. The study found that when the influent salinity was increased to 15 g·L^-1 and 30 g·L^-1, the nitrogen removal performance of the reactor decreased slightly, but was restored with the extension of the running time. After the performance of the reactor became stable, the abundance of the ANAMMOX bacteria under the three salinity conditions was 10.33%, 20.90%, and 35.87%, of which Candidatus Kuenenia was the dominant genus. Planctomycetes, Proteobacteria, and Chloroflexi accounted for a high proportion in the granular sludge, and their cumulative abundance exceeded 80%, making them the dominant phyla of the reactor. Under saline conditions, the abundance of the Planctomycetes bacteria increased, the abundance of the Proteobacteria bacteria decreased, and the abundance of the Chloroflexi bacteria was relatively stable. Electron microscopy showed a large number of filamentous bacteria and extracellular polymers were present on the surface of the granular sludge under saline conditions. The increased abundance of the denitrifying bacteria enhanced the cooperative denitrification, the presence of aerobic microorganisms and denitrifying bacteria facilitated the maintenance of anaerobic conditions inside the reactor, and the increased abundance of Chloroflexi and Bacteroidetes was beneficial to maintain the stability of the granular sludge structure. These results showed that the ANAMMOX bacteria could be adapted to saline conditions by acclimation, and that the associated bacteria provided support for the function of the ANAMMOX bacteria under saline conditions.

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