中文关键词全程自养脱氮工艺一体式反应器微生物菌群结构城市污水厌氧氨氧化 英文关键词completely autotrophic nitrogen removal over nitrite(CANON)integrated reactorstructure of microbial communityurban sewageanaerobic ammonia oxidation

作者	单位	E-mail
李田	苏州科技大学环境科学与工程学院, 苏州 215009	lt690824185@sina.com
印雯	苏州科技大学环境科学与工程学院, 苏州 215009
王昕竹	苏州科技大学环境科学与工程学院, 苏州 215009
沈耀良	苏州科技大学环境科学与工程学院, 苏州 215009 江苏省水处理技术与材料协同创新中心, 苏州 215009 江苏省环境科学与工程重点实验室, 苏州 215009
吴鹏	苏州科技大学环境科学与工程学院, 苏州 215009 江苏省水处理技术与材料协同创新中心, 苏州 215009 江苏省环境科学与工程重点实验室, 苏州 215009	wupengniu@126.com
宋吟玲	苏州科技大学环境科学与工程学院, 苏州 215009 江苏省水处理技术与材料协同创新中心, 苏州 215009 江苏省环境科学与工程重点实验室, 苏州 215009

中文摘要 CANON工艺如能处理低氨氮城市生活污水，将大幅度降低市政污水处理能耗.故以纤维载体为填料，在CSTR反应器中同时接种亚硝化污泥和厌氧氨氧化污泥启动CANON反应器，且在CANON系统前端添加ABR除碳系统，构建ABR除碳-CANON耦合工艺，研究ABR除碳-CANON耦合工艺除碳脱氮性能，并采用MiSeq高通量测序技术分析污泥中微生物菌群结构的变化情况.结果表明，通过同时接种亚硝化污泥和厌氧氨氧化污泥，控制DO为0.5~2 mg·L^-1、HRT为6h、pH值为8左右等措施，在55 d内成功启动CANON系统，TN去除率为81%~87%，氨氮负荷为0.195 kg·（m³·d）^-1.ABR除碳系统出水有机物浓度（120 mg·L^-1）不会对后续CANON系统产生不利影响，一体式ABR除碳-CANON工艺TN去除率在74%~87%，出水COD平均浓度为40 mg·L^-1.同时，CANON系统启动后变形菌门（Proteobacteria）得到了显著提升，鞘脂杆菌纲（Sphingobacteria）所占比例下降为6.8%，CANON系统中亚硝化菌和厌氧氨氧化菌不断淘汰劣势菌群成为反应器内优势菌群，一体化ABR除碳-CANON工艺对城市污水具有良好的脱氮除碳效果. 英文摘要 If municipal wastewater can be treated by the completely autotrophic nitrogen removal over nitrite (CANON) process, it will greatly reduce the energy consumption of municipal wastewater treatment. The CANON reactor with a fiber carrier was started up by seeding nitrosation sludge and anaerobic ammonia oxidation (ANAMMOX) sludge in the continuously stirred tank reactor (CSTR). An ABR decarbonization system was added to the front of the CANON system to build the ABR decarbonization-CANON coupling process to examine carbon and nitrogen removal characteristics of the whole system. The high throughput sequencing technology of MiSeq was also employed to analyze the structure of the microbial community before and after the reactivation. The results showed that mixing nitrosation sludge and ANAMMOX sludge in the CSTR reactor under controlled parameters (DO of 0.5-2 mg·L^-1; HRT for 6 h; pH of 8) allowed the CANON system to successfully start within 55 d, with a TN removal rate of 81%-87% and ammonia nitrogen load of 0.195 kg·(m³·d)^-1. The effluent COD concentration of the ABR decarbonizing system did not adversely affect the subsequent CANON system, and the TN removal rate of the ABR decarbonization-CANON process was between 74% and 87%. Additionally, the average concentration of COD in the effluent was 40 mg·L^-1. At the same time, the Proteobacteria gate significantly improved after the CANON system began, and the proportion of Sphingobacteria decreased to 6.8%. Nitrifying bacteria and anaerobic ammonia oxidizing bacteria in the CANON system continuously eliminated the inferior bacterial groups to become the dominant group in the reactor. The integrated ABR decarbonization-CANON process had a positive effect on the denitrification and decarbonization of urban sewage.

PDF全文下载地址:

https://www.hjkx.ac.cn/hjkx/ch/reader/create_pdf.aspx?file_no=20190238&flag=1&journal_id=hjkx&year_id=2019