中文关键词
黄山细菌群落结构Illumina高通量测序土壤 pH土壤酶活性 | 英文关键词Mount Huangshanbacterial community structureIllumina high-throughput sequencingsoil pHsoil enzyme activity |
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| 中文摘要 |
| 黄山具有保存较完整的生态系统及明显的地势高差，为研究中亚热带森林生态系统土壤微生物群落海拔分布格局提供天然场所.本研究在黄山景区670~1870 m按每100 m的间隔采集土样，利用Illumina MiSeq高通量测序技术分析土壤细菌群落结构及多样性的海拔变化特征，同时测定相关土壤理化性质和土壤酶活性.结果表明：①土壤全氮、碱解氮、全钾和总有机碳含量均存在显著海拔差异（P<0.01），且总体上随着海拔升高而增加；土壤蔗糖酶活性存在显著海拔差异（P<0.01），且总体上随海拔升高而增强，但酸性磷酸酶和脲酶活性无显著海拔分异（P>0.05）；②将12个海拔梯度分为高中低3组（低海拔670~875 m；中海拔1080~1370 m；高海拔1460~1780 m），发现土壤细菌OTU数目：中海拔>低海拔>高海拔，但是高、中、低海拔的差异不明显；③在875~1370 m小范围海拔内，土壤细菌群落多样性沿海拔呈单峰模式分布；而在670~1780 m整个海拔范围内，土壤细菌群落多样性无明显海拔分布模式；④所有样地中，相对丰度大于3%的优势菌门共7个，优势菌目共15个；⑤相关性热图分析表明，土壤pH对不同海拔土壤细菌群落结构差异性影响最大.Pearson相关性分析和偏Mantel分析表明，细菌群落α多样性（P<0.01）和β多样性（偏Mantel r=0.560，P=0.001）主要受土壤pH影响.因此，土壤pH是决定黄山不同海拔土壤细菌群落结构及多样性的主要环境因子. |
| 英文摘要 |
| Mount Huangshan has a well-preserved ecosystem and obvious differences in vertical geography, which provide a natural laboratory for studying the altitudinal distribution patterns of soil microbial communities in a mid-subtropical forest ecosystem. The soil bacterial community structure and diversity of the samples collected every 100 m from 670 to 1870 m on the south slope of Mount Huangshan were examined using Illumina MiSeq high-throughput sequencing technology. The soil physicochemical properties and soil enzyme activities of the samples were also measured to explore the relationship between bacterial communities and soil properties as well as enzyme activities. The results showed that ① The contents of soil total nitrogen, available nitrogen, total potassium, and total organic carbon were significantly different across the altitudes (P<0.01) and generally increased as altitude increased. The soil sucrase activities across altitudes were significantly different (P<0.01), and generally increased as altitude increased. However, there was no significant difference in acid phosphatase and urease activities between different altitudes (P>0.05). ② The 12 elevational gradients were divided into three groups:low altitude (670-875 m), medium altitude (1080-1370 m), and high altitude (1460-1780 m). The OTUs in low altitude sites were greater than in high altitude sites but lower compared to medium altitude sites. However, the differences in OTUs across altitude sites were not significant. ③ The soil bacterial community diversity showed a unimodal pattern in a small range of altitudes from 875-1370 m, although no apparent trend was observed at the altitudes from 670-1780 m. ④ There were 7 dominant phyla and 15 dominant orders with a relative abundance of more than 3% in all soil samples. ⑤ Correlation heat map analysis between the top 15 bacterial phyla and soil physicochemical properties as well as enzyme activities showed that soil pH had the greatest effect on the differences in soil bacterial community structure across the different altitudes. Pearson correlation analysis and Partial Mantel test also showed that bacterial community α-diversity (P<0.01) and β-diversity (Partial Mantel r=0.560, P=0.001) were mainly affected by soil pH. Consequently, soil pH was the key environmental factor determining the soil bacterial community structure and diversity across the different altitudes on Mount Huangshan. |
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