中文关键词石油污染土壤微生物修复高通量测序微生物群落结构多样性 英文关键词petroleum contaminated soilbioremediationhigh throughput sequencingmicrobial community structurebiodiversity

作者	单位	E-mail
祁燕云	西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055	1218750691@qq.com
吴蔓莉	西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055	447005853@qq.com
祝长成	西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055
叶茜琼	西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055
徐会宁	西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055

中文摘要 利用高通量测序技术对微生物修复石油污染土壤过程中的微生物群落结构变化进行研究.结果表明，经修复处理的土壤微生物群落结构及多样性发生明显变化.利用生物强化修复处理（BA）的土壤中，微生物丰富度与均匀度明显降低，土著菌群受到抑制，外加变形菌门（Proteobacteria）成为主要的优势菌门，相对丰度由修复前的37.44%增加为87.44%.假单胞菌属（Pseudomonas）成为土壤中的优势菌属，丰度由2.99%增加为76.37%；进行生物刺激修复处理的土壤（BS）菌群丰富度和均匀度与原污染土壤相比略有降低.菌群结构组成上，原优势菌门变形菌门（Proteobacteria）丰度由37.44%降低为10.90%，厚壁菌门（Firmicutes）丰度由9.16%增加为35.32%，属水平上，原优势菌属微小杆菌属（Exiguobacterium）和原小单胞菌属（Promicromonospora）丰度由8.49%和18.96%分别降低为2.19%和14.97%，诺卡氏菌属（Nocardioides）和芽孢杆菌属（Bacillus）丰度由5.56%和0.29%分别增加至28.95%和22.70%，成为主要优势菌属.生物强化修复处理引起土壤菌群多样性和结构发生明显变化，生物刺激修复处理可基本保持土壤菌群结构多样性不被破坏，土壤菌群结构的稳定有利于石油烃的生物降解. 英文摘要 The shift in microbial community structure during the bioremediation of oil-polluted soil was analyzed by high-throughput sequencing. The results demonstrated obvious changes in the soil microbial community structure and diversity during bioremediation. The species richness and evenness of the microbial community decreased substantially due to the bioaugmentation treatment. Proteobacteria became the predominant phylum, with a relative increase in abundance from 37.44% to 87.44%. Pseudomonas was the most dominant genus, which increased in abundance from 2.99% to 76.37%. In the biostimulation treated soil, the relative abundance of Proteobacteria decreased from 37.44% to 10.90%, while the phylum Firmicutes increased from 9.16% to 35.32%. At the genus level, the relative abundances of Exiguobacterium and Promicromonospora decreased from 8.49% and 18.96% to 2.19% and 14.97%, respectively. Nocardioides and Bacillus became the dominant genera and increased from 5.56% and 0.29% to 28.95% and 22.70%, respectively. The results indicated that bioaugmentation substantially influenced the soil microbial diversity and community structure. Additionally, the biostimulation treatment maintained the balance in the soil microbial community structure. The stabilization of bacteria community structure is beneficial to petroleum biodegradation in the soil.

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