中文关键词生物处理污泥沉降性能菌群结构胞外聚合物荧光特性 英文关键词biological treatmentsludge settleabilitymicrobial community structureextracellular polymeric substancesfluorescence characteristics

作者	单位	E-mail
温丹丹	西安建筑科技大学, 陕西省环境重点实验室, 西北水资源环境与生态重点实验室, 西安 710055	781224035@qq.com
袁林江	西安建筑科技大学, 陕西省环境重点实验室, 西北水资源环境与生态重点实验室, 西安 710055	yuanlinjiang@xauat.edu.cn
陈希	西安工程大学环境与化学工程学院, 西安 710048
王洋	西安建筑科技大学, 陕西省环境重点实验室, 西北水资源环境与生态重点实验室, 西安 710055
申童童	西安建筑科技大学, 陕西省环境重点实验室, 西北水资源环境与生态重点实验室, 西安 710055
刘小博	西安建筑科技大学, 陕西省环境重点实验室, 西北水资源环境与生态重点实验室, 西安 710055

中文摘要 均以乙酸钠为碳源，考察了AAO脱氮除磷（Ⅰ阶段）、AO脱氮（Ⅱ阶段）、好氧除碳（Ⅲ阶段）这3种工艺下污泥沉降性能的变化，追踪了污泥中微生物群落结构的演替、监测了微生物代谢产物的含量及组分变化，分析运行方式对污泥沉降性能变化的影响.结果表明，Ⅰ阶段沉降性能最佳，其次为Ⅲ、Ⅱ阶段.运行条件的不同，系统优势菌及菌群结构都发生了显著变化，其中Thiothrix的相对数量是影响污泥沉降性能变化的主导细菌.在接种污泥和Ⅰ阶段Thiothrix的丰度仅为0.08%和1.51%，Ⅱ阶段上升至9.41%，Ⅲ阶段降至4.29%.Ⅰ阶段的厌氧区对该菌的生长具有抑制作用，但Ⅱ阶段的缺氧区却刺激其优势生长.同时对比了3个系统中微生物种群多样性，Ⅰ阶段最高，其次为Ⅱ、Ⅲ阶段.缺氧区和厌氧区的引入导致系统功能与环境的复杂度增加，微生物群落多样性有所升高.胞外聚合物（extracellular polymeric substances，EPS）中各组分含量及三维荧光测定结果表明，微生物群落结构变化对EPS的组分及含量具有显著影响，进而导致污泥沉降性能改善或恶化的进程加剧，污泥沉降性能与松散附着胞外聚合物（loosely bound EPS，LB-EPS）中蛋白质/多糖的比值呈正相关. 英文摘要 Using sodium acetate as the carbon source, sludge settling ability (settleability) was investigated under three processes:AAO nitrogen and phosphorus removal(process Ⅰ), AO nitrification-denitrification (process Ⅱ), and aerobic carbon removal (process Ⅲ). The succession of microbial community structures in sludge was traced, the content and composition of microbial metabolites were monitored, and the effects of operational mode on sludge settleability were analyzed. The results showed that the settleability of process Ⅰ was the best, followed by process Ⅲ and Ⅱ. Under the different operating conditions, the dominant bacteria and microbial community structure of the system changed significantly. The relative amount of Thiothrix was the dominant bacteria affecting the sludge settleability. The abundances of Thiothrix were only 0.08% and 1.51% with fresh sludge and in process Ⅰ; this abundance increased to 9.41% in process Ⅱ and decreased to 4.29% in process Ⅲ. The anaerobic zone of process I had an inhibitory effect on the growth of the bacterium, while the anoxic zone of process Ⅱ stimulated its dominant growth. At the same time, comparison showed that the microbial population diversity was highest in process Ⅰ. followed by processes Ⅱ and Ⅲ. The introduction of anoxic and anaerobic zones led to the increase of system function and environmental complexity, and increased microbial community diversity. Analyses of extracellular polymeric substances (EPS) and fluorescence characteristics showed that the changes in microbial community structure had a significant effect on the composition and content of EPS, which aggravated the process of improving or deteriorating settleability. The sludge settleability was found to be positively correlated with the ratio of protein and polysaccharide in loosely bound EPS.

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