中文关键词再生水土柱灌水水平酶活性耐热大肠菌群 英文关键词reclaimed watersoil columnirrigation levelenzyme activitythermotolerant coliforms

作者	单位	E-mail
韩洋	中国农业科学院农田灌溉研究所, 新乡 453003 中国农业科学院研究生院, 北京 100081	1515507632@qq.com
李平	中国农业科学院农田灌溉研究所, 新乡 453003 农业部农产品质量安全水环境因子风险评估实验室, 新乡 453003
齐学斌	中国农业科学院农田灌溉研究所, 新乡 453003 农业部农产品质量安全水环境因子风险评估实验室, 新乡 453003	qxb6301@sina.cn
崔丙健	中国农业科学院农田灌溉研究所, 新乡 453003 农业部农产品质量安全水环境因子风险评估实验室, 新乡 453003
常迪	中国农业科学院农田灌溉研究所, 新乡 453003 中国农业科学院研究生院, 北京 100081
马欢欢	中国农业科学院农田灌溉研究所, 新乡 453003 中国农业科学院研究生院, 北京 100081

中文摘要 为了探讨再生水不同灌水水平对土壤酶活性和环境污染指示菌耐热大肠菌群分布的影响，采用室内土柱灌水实验，研究再生水、自来水不同灌水水平对土壤酶活性和耐热大肠菌群分布的影响.结果表明：①在土壤酶活性方面，相同灌水水平下，与自来水处理相比，再生水灌溉处理提高了土壤脲酶和蔗糖酶活性；再生水不同灌水水平对比分析表明，充分灌水处理显著提高了土壤蔗糖酶活性和深层土壤过氧化氢酶活性.②在土壤耐热大肠菌群分布方面，相同灌水水平下，与自来水灌溉处理相比，再生水灌溉显著提高了土壤耐热大肠菌群数量；再生水不同灌水水平对比分析表明，充分灌水处理显著增加了土壤耐热大肠菌群数量；4个处理的表层土壤耐热大肠菌群数量均显著高于深层，且随着灌溉时间的推移，0~60 cm各土层的土壤耐热大肠菌群数量存在降低趋势.③通过对土壤耐热大肠菌群数量与土壤酶活性的相关性分析表明，土壤耐热大肠菌群数量与土壤脲酶和蔗糖酶活性呈极显著正相关，与土壤过氧化氢酶活性呈显著正相关.因此，再生水灌溉促进了土壤C、N循环转化酶的活性，合理控制再生水灌水水平可以有效阻控土壤耐热大肠菌群的数量. 英文摘要 This study aimed to discern the effect of different levels of irrigation with reclaimed water on soil enzyme activities and on the distribution of thermotolerant coliforms. The effects of two irrigation water qualities (reclaimed water and tap water) and two irrigation levels (full irrigation and insufficient irrigation) on soil enzyme activity and distribution of thermotolerant coliforms were studied through indoor soil column irrigation. Results indicated that:① Reclaimed water irrigation increased soil urease and invertase activity compared with tap water under the same irrigation conditions. Compared with insufficient irrigation with reclaimed water, full irrigation with reclaimed water significantly increased soil invertase activity and deep soil catalase activity. ② At the same irrigation level, the number of thermotolerant coliforms in soil significantly increased with reclaimed water irrigation. The number of thermotolerant coliforms in soil under full irrigation with reclaimed water was significantly higher than with insufficient irrigation using reclaimed water. The number of thermotolerant coliforms in surface soil of the four irrigated treatments was significantly higher than in the deep soil layer, and with progression of irrigation, the number of thermotolerant coliforms in the 0-60 cm soil layer decreased. ③ There was a significant positive correlation between the quantity of thermotolerant coliforms and the activity of soil urease and invertase, and between the quantity of thermotolerant coliforms and the activity of soil catalase. Reclaimed water therefore promoted enzyme activity that can promote transformation of C and N in soil. Reasonable irrigation with reclaimed water can therefore effectively control the number of thermotolerant coliforms in soil.

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