摘要:以"陇春27"号水培小麦幼苗为研究材料，外源添加水杨酸（SA）、一氧化氮（NO）清除剂（carboxy-PTIO，c-PTIO）、NO供体硝普钠（SNP）、硝酸还原酶（NR）抑制剂钨酸盐（Tungstate）及NO合成酶（NOS）抑制剂（L-NAME）进行不同预处理，分析其在镉（Cd）胁迫下根的生长和叶片叶绿素含量的变化，探讨SA和NO互作对小麦幼苗Cd胁迫的缓解机制。结果表明：随着Cd处理时间的延长，小麦幼苗根中SA含量显著降低，NO含量则呈现先增加（6 h和12 h）后减少（24 h和48 h）的趋势；Cd胁迫抑制了小麦幼苗根的生长，减少了叶片叶绿素的含量，而一定浓度的SA或SNP预处理可以缓解Cd胁迫对小麦幼苗根长的抑制作用，增加叶绿素的含量。c-PTIO、L-NAME和Tungstate单独预处理显著抑制了小麦幼苗根的生长，减少了NO的含量，但不影响叶绿素含量。SA400+L-NAME预处理可以缓解Cd胁迫对小麦幼苗根长的抑制作用以及叶绿素和NO含量的减少作用；SA400+c-PTIO或SA400+Tungstate预处理可增加Cd胁迫下叶绿素的含量，但对根的伸长无影响。进一步研究发现，Cd胁迫抑制了NR的活性，而SA400预处理可以使Cd胁迫下NR的活性增强，不同处理对NOS的活性均无影响。综上所述，Cd胁迫导致小麦幼苗根内源SA含量降低和NO含量先升高再降低；外源添加SA或SNP预处理缓解了Cd胁迫对根生长的抑制和叶绿素含量降低的作用；外源SA通过影响NO的产生从而提高小麦幼苗对Cd胁迫的耐受性，最终缓解了Cd对小麦幼苗的毒害作用。



Abstract:Soil pollution of heavy metals is very severe in China, especially the cadmium pollution. Excessive cadmium not only causes severe damage to the growth and development of plants, leading to disorders of their physiological functions, reduced photosynthesis, and reduced organic matter accumulation and so on, but also greatly endangers human health. In this research, the growth of roots and the content of chlorophyll in the seedlings of wheat (Triticum aestivum L. cv. Longchun 27) were analyzed after being pretreated with exogenous salicylic acid (SA), nitric oxide (NO) scavenger (Carboxy-PTIO, c-PTIO), NO donor sodium nitratol (SNP), nitrate reductase (NR) inhibitor (Tungstate), NO synthase inhibitor (L-NAME) and then being treated with 100 μmol/L CdCl₂, to explore the mitigation mechanism of SA and NO interaction on Cd-induced phytotoxicity. The results showed that with the prolongation of cadmium treatment time, the content of SA decreased gradually, when the treatment time was extended to 24h, the SA content no longer changed, but the content of NO increased firstly (6 h and 12 h), then decreased (24 h and 48 h) in the roots of wheat seedlings. Cd stress significantly inhibited the root growth and reduced the chlorophyll content of the leaves. However, pretreatment with suitable SA or SNP could moderate the inhibition of Cd stress on the growth of roots and increase the content of chlorophyll in wheat seedlings. The single pretreatment of c-PTIO, L-NAME and Tungstate restrained the growth of roots significantly and reduced the content of NO, but had little effect on the content of chlorophyll. The pretreatment of SA400+L-NAME could alleviate the inhibition of root elongation and the reduction of chlorophyll as well as NO content in wheat seedlings were induced by Cd stress; the pretreatment SA400+c-PTIO or SA400+Tungstate could augment the content of chlorophyll, but there was no effect on root elongation under Cd stress. Further studies showed that Cd stress significantly weaken NR activity, while the pretreatment of SA400 could increase the NR activity of wheat seedlings roots under Cd stress; NOS activity was not affected by different treatments. In summary, Cd stress caused the decrease of endogenous SA content and the first increase of NO content and then decrease of wheat seedlings; the pretreatment with exogenous SA or SNP reduced the inhibition of root growth and the decrease of chlorophyll content by Cd stress; exogenous SA improved the tolerance of wheat seedlings to Cd stress by affecting the production of NO, ultimately, the phytotoxic effects of Cd on wheat seedlings were alleviated.





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