摘要:冰川棘豆是青藏高原高寒草原和荒漠草原主要伴生毒草之一，对青藏高原草地退化及畜牧业的发展产生严重影响。对4种不同生态环境中冰川棘豆根系土壤细菌的16S rRNA进行高通量测序及生物信息学分析，结合土壤的理化性质，分析了不同生态环境下冰川棘豆根系土壤细菌群落多样性及其与土壤理化因子的关系，探讨了根系土壤细菌对冰川棘豆在高寒生态系统中形成优势种可能的影响。结果表明，在不同生态环境下冰川棘豆根系土壤细菌多样性丰富，且受土壤pH和有机物影响最大，同时，能够保持稳定的核心菌群网络，对冰川棘豆在高寒生态系统中形成优势种具有促进作用。



Abstract:Oxytropis glacialis is one of the main poisonous weeds in the alpine grassland and desert grassland of the Qinghai-Tibet Plateau (QTP). It has a serious impact on the grassland degradation and the development of animal husbandry in the QTP. In this paper, the bacterial 16S rRNA in the root system soil of Oxytropis glacialis was sequenced by high-throughput sequencing and analyzed by bioinformatics methods. Combining with the physical and chemical properties of soil samples, the bacterial diversity and its relationship with soil physical and chemical factors was analyzed. The possible influence on root system soil bacteria of Oxytropis glacialis to form dominant species in alpine ecosystem was discussed. The results showed that the diversity of root system soil bacteria of Oxytropis glacialis was rich in different ecological environments, and was most affected by soil pH and organic matter. Meanwhile, the stable core microbiota network could be maintained, which could promote the formation of Oxytropis glacialis becoming dominant species in the alpine ecosystem.





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