摘要:为探讨温度升高和外源碳输入对泥炭地土壤碳氮循环关键微生物的影响，于2017年7月采集多年冻土区泥炭地表层（0-10 cm和10-20 cm）土壤样品，在10、15℃两个温度下开展为期42d的增温模拟试验，同时设置葡萄糖添加处理，利用荧光定量PCR技术分析泥炭地土壤碳氮循环关键微生物丰度变化，同时分析增温和外源碳输入对泥炭地土壤活性碳组分和无机氮含量的影响。结果表明：温度升高可导致北方泥炭地表层土壤微生物丰度以及群落结构变化，0-10 cm土壤微生物比10-20 cm土壤微生物更加敏感。增温条件下微生物首先快速分解活性有机碳，同时温度升高加快土壤氮周转速率，增加有效氮含量。外源碳输入整体提高了深层土壤微生物丰度，使得10-20 cm土壤细菌、产甲烷菌、甲烷氧化菌、氨氧化细菌以及反硝化细菌丰度显著增加，说明外源碳输入可能会促进10-20 cm土壤甲烷氧化过程、氨氧化过程和反硝化过程。温度和葡萄糖的交互作用对泥炭地表层土壤碳氮循环关键微生物丰度均有显著影响。在增温和外源碳输入条件下，北方泥炭地表层土壤微生物丰度受土壤碳氮活性基质的影响。



Abstract:In order to investigate the effects of temperature rise and exogenous carbon input on key microorganisms of carbon and nitrogen cycling in peatland soil, surface soil samples (0-10 cm and 10-20 cm) of peatland in permafrost region were collected in July 2017. The simulation experiment of temperature increasing was conducted for 42 days under 10 ℃ and 15 ℃, and glucose addition treatment was set up. Fluorescence quantitative PCR technology was used to analyze the changes of key microbial abundances in soil carbon and nitrogen cycles in peatlands. Simultaneously analyze the effects of warming and exogenous carbon input on soil activated carbon components and inorganic nitrogen content in peatlands. The results showed that the increasing of temperature could change the abundance and community structure of soil microorganisms in northern peatlands soil. Soil microorganisms in 0-10 cm soils were more sensitive than those in 10-20 cm. Under the condition of increasing temperature, microorganisms rapidly decomposed active organic carbon. Meanwhile, the increasing of temperature accelerated the turnover rate of soil nitrogen and increased the available nitrogen content. Exogenous carbon input overall improved the microbial abundance of deep soil, which significantly increased the abundance of bacteria, methanogens, methanotrophores, ammoxides and denitrifiers in 10-20 cm soil, and hence possibly promoted the process of methane oxidation, ammonia oxidation and denitrification in 10-20 cm soil. There was a significant interactive effect of temperature increasing and glucose addition on the abundance of key microorganisms in soil carbon and nitrogen cycle in peatland surface soil. Under the conditions of increasing temperature and exogenous carbon input, the abundance of microorganism in northern peatlands surface soil was influenced by the availability of active carbon and nitrogen substrates.





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