摘要:为研究黄河三角洲地区混交人工林土壤细菌群落特征，应用高通量测序技术，比较分析了刺槐臭椿混交林以及臭椿和刺槐纯林土壤细菌结构及多样性，并结合土壤理化性质进行分析。试验结果表明：在细菌门分类水平上，臭椿纯林、刺槐纯林、刺槐臭椿混交林土壤中分别检测出27、25、31门细菌，3种不同林分土壤中酸杆菌门、变形菌门、放线菌门、硝化螺旋菌门、绿弯菌门、浮霉菌门、芽单胞菌门、疣微菌门8种细菌是土壤中的主要细菌群落，其中酸杆菌门、变形菌门和放线菌门为优势细菌群落。不同类型人工林土壤中各门细菌相对丰度差异显著。混交林土壤细菌物种数和Shannon指数值分别为1910和9.1高于两种纯林。通过对土壤主要细菌群落与土壤理化性质进行主成分分析发现，3种不同林分之间在土壤细菌群落结构上有较高程度的分离，差异显著（P < 0.05），有效磷含量与混交林土壤细菌群落有较强的正相关关系。因此可以得出结论，不同林分类型、土壤理化性质和细菌群落结构三者相互影响，刺槐臭椿混交增加了土壤细菌群落多样性，土壤理化性质在一定程度上影响土壤细菌结构和多样性。



Abstract:The aim of the present study was to investigate the characteristics of soil bacterial community in different forests in the Yellow River Delta. High-throughput sequencing technology was used to compare the soil microbial communities from different forests, that is pure Robinia pseudoacacia forest, pure Ailanthus altissima forest, and their mixed forest. The soil physicochemical properties were measured to determine the correlations between them. Results showed that the composition and structure of soil bacterial communities were different among these forests. At the level of bacterial phylum classification, 27, 25, 31 bacteria were detected in the soil of A. altissima, R. pseudoacacia, and the mixed forest, respectively. For all these three stands, there were eight main bacterial communities, that was acidobacteria, proteobacteria, actinobacteria, nitrospirae, chloroflex, planctomycetes, gemmatimonadetes and verrucomicrobia, among which the acidobacteria, proteobacteria and actinobacteria were the dominant bacterial communities. The relative abundance of bacteria in the mixed plantations were significantly different from that in the pure plantations. Besides, the observed speices (1910) and Shannon index (9.1) were highest in the mixed forests. Through principal component analysis (PCA) we found that there were significant separation for soil bacterial community structure among the three different stands(P < 0.05). And for mixed forest, there was a strong positive correlation between the available phosphorus and soil bacterial community. Therefore, different stand types, soil characteristics and cummunity structures intereact with each other. The mixed plantations could increase the diversity of soil bacterial community. And the soil bacterial structure and diversity could be affected by the soil physical and chemical properties.





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