摘要:在人工气候箱内，通过设置不同水深梯度（0 cm；3 cm；6 cm）和光照强度（标准光照：400 μmol m^-2 s^-1；低光照：240 μmol m^-2 s^-1），对长喙毛茛泽泻生物量、叶型特征和生理特征的影响，探讨长喙毛茛泽泻对不同水深和光照强度的适应性以及其濒危原因。结果表明：在同一光照强度下长喙毛茛泽泻叶生物量、茎生物量、根生物量、总生物量、叶宽、叶面积、过氧化物酶（POD）活性、可溶性蛋白和可溶性糖都随水深的增加呈先升高后降低的趋势；丙二醛（MDA）随水深的增加呈先降低后升高的趋势；叶柄长度与水深呈正相关；脯氨酸含量与水深呈负相关。在同一水深条件下，光照强度的降低显著降低了叶生物量、茎生物量、总生物量、过氧化物酶（POD）活性、可溶性蛋白、可溶性糖和脯氨酸的含量。由此可知，水深的变化和光照不足都将导致长喙毛茛泽泻生长减弱，且长喙毛茛泽泻对这些不利条件的适应能力较弱。剧烈的水深变化和上层植物的荫蔽可能是导致其濒危的重要原因。



Abstract:In an artificial climatic cabinet, Ranalisma rostratum was subjected to three water depths which were 0 cm, 3 cm, and 6 cm, and two light intensity treatments which were 400 μmol m^-2 s^-1 and 240 μmol m^-2 s^-1. By recording the biomass, foliar morphological characteristics, and other physiological characteristics, the adaption of R. rostratum to different water depths and light intensities, and the reason why the plant is endangered were concluded. Under the same light intensity, with increasing water depth, proline content decreased; petiole length increased; leaf biomass, stem biomass, root biomass, total biomass, leaf width, leaf area, peroxidase activity, soluble protein, and soluble sugar content initially increased but subsequently decreased; and malondialdehyde content initially decreased but subsequently increased. At the same water depth, low-light intensity stress decreased leaf biomass, stem biomass, total biomass, peroxidase activity, soluble protein, soluble sugar, and proline content. It can be concluded that lack of light intensity and a change in water depth can all lead to R. rostratum's dysplasia and the plant cannot adapt well to these adverse conditions. Therefore, in the wild, sharp changes in water depth and the shade from upper plants may be vital reasons for the endangerment of R. rostratum.





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