摘要:为了充分利用米槁林下空间，从化感效应的角度研究人工米槁林下土壤水浸提液对绿壳砂生长状况的影响，探索米槁与绿壳砂建立间作经营系统的可能性。以绿壳砂为受体，米槁根际土壤与林间土壤水浸提液为供试液，设置6个浓度0、0.5、1、5、10、50 mg/mL的水浸提液处理绿壳砂，对绿壳砂种子萌发和幼苗生理生化指标进行测定，以此探讨米槁根际土壤与林间土壤水浸提液对绿壳砂生长的影响。结果表明：米槁两种土壤水浸提液对绿壳砂种子的萌发大体上呈现低浓度促进高浓度抑制的浓度效应，但随着浓度的变化，差异不显著；米槁林间土壤水浸提液对绿壳砂幼苗的总生物量及苗高都表现出显著的促进作用，呈先增加后缓慢减小的趋势，根际土壤水浸提液则在高浓度时表现出抑制作用。从化感效应综合指数看出，2种水浸提液对可溶性蛋白质含量、游离脯氨酸（Pro）含量、土壤脲酶（S-UE）活性表现为抑制作用，其余都为促进作用，且林间土壤的促进效果强于根际土壤。应用GC-MS从米槁根际土壤与林间土壤中分别鉴定出18种化合物，虽含有酚酸和烷烃类等化感物质，但在一定浓度范围内，对绿壳砂种子萌发、幼苗生长没有明显的抑制作用，且在米槁林土水浸提液处理下表现出更强的适应性。因此，在米槁林下套种绿壳砂时采取幼苗移栽法，构建米槁-绿壳砂林药间作经营体系具有一定可行性。



Abstract:To make full use of the space under the Cinnamomum migao forest, the effects of soil water extracts in an artificial C. migao forest on the growth of Amomum villosum were studied from the perspective of the allelopathic effect, and the possibility of establishing an intercropping system for C. migao and A. villosum was explored. A. villosum as the acceptor, and the rhizosphere and forest soil water extracts were used as the test solution, six water extracts at concentrations of 0, 0.5, 1, 5, 10, 50 mg/mL were used to treat A. villosum, and the germination of the A. villosum seeds, the growth of the seedlings, and the soil enzyme activities were studied.We also investigated the effects of C. migao soil rhizosphere soil and forest soilwater extract on the growth of A. villosum. The results showed that the germination of A. villosum extracts by the two soil water extracts of C. migao were inhibitory at high concentrations, but stimulatory under low concentrations, with no significant differences. Forest soil of C. migao water extract had a significant effect on the total biomass and seedling height of the A. villosum seedlings; it increased first and then slowly decreased. The rhizosphere soil water extract was inhibiting at high concentrations. Thus, based on the synthetic allelopathic index, the two aqueous extracts have inhibitory effects on soluble protein content, free proline (Pro) content, and soil urease (S-UE) activity, and the rest are promoting effects. The promoting effects of forest soil are stronger than the rhizosphere soil. The GC-MS was used to identify 18 compounds from the rhizosphere and forest soils of C. migao. However, it contained allelochemicals such as phenolic acids and alkanes, it had no obvious inhibitory effect on seed germination and seedling growth of A. villosum over a certain concentration range, and it showed stronger adaptability under the treatment of forest soil of C. migao water extracts. Therefore, it is possible to adopt the seedling transplanting method when planting A. villosum under the C. migao forest and constructing the forest-medicinal herb intercropping system of the C. migao-A. villosum.





PDF全文下载地址:

https://www.ecologica.cn/stxb/article/pdf/stxb201807311631