摘要:土壤微生物在森林生态系统中起着重要作用。为了了解木论喀斯特常绿落叶阔叶混交林土壤细菌的多样性，明确采样数对多样性的影响，采用Illumina Hiseq高通量测序技术对木论喀斯特常绿落叶阔叶混交林土壤细菌多样性进行了初步研究，发现：在82个采样点内，随着采样点增加，不同分类水平的土壤细菌类群数量有增多趋势；当采样点达到82个时，检测出的土壤细菌类群达44门99纲180目356科630属和269822个OTU；82个土壤样品中所检测出的细菌类群平均有28.8门60.9纲108.1目206.8科239.6属和3290.5个OTU，其中门、纲、目分类水平上优势类群所占比例分别为变形菌门（Proteobacteria）（34.51%）、α-变形菌纲（Alphaproteobacteria）（18.43%）、根瘤菌目（Rhizobiales）（12.50%）。取样数量对检测出的土壤细菌类群数量有显著影响：在门、纲、目、科的分类水平上，36个、57个、72个、78个样品基本上可代表82个样品监测出来不同分类水平的细菌多样性信息；在属和OTU的分类水平上，当样品数量达到82个时检测出来的相应细菌类群数仍在不断地增加。这些初步的研究结果显示木论常绿落叶阔叶混交林土壤在一定程度上有着较高的细菌多样性水平，该研究为细菌多样性与植物多样性的进一步研究奠定了基础。



Abstract:Soil microbes play essential roles in forest ecosystems. In this study, we investigated soil bacterial diversity in the Mulun karst evergreen and deciduous broad-leaved mixed forest using the Illumina Miseq platform. The results showed that in the 82 soil sampling points, the number of bacterial taxa detected at different classification levels increased with increasing number of sampling points, and the degree of increase differed. When the sampling points were 82, the mean values of relative bacterial taxa in the 82 samples were 28.8 phyla, 60.9 classes, 108.1 orders, 206.8 families, 239.6 genera, and 3290.5 operational taxonomic units (OTU). At the classification level of phylum, class, and order, the dominant groups were Proteobacteria (34.51%), α-Alphaproteobacteria (18.43%), and Rhizobiales (12.50%), respectively. The number of samples has a significant effect on the number of soil bacteria group. At the classification level of phylum, class, order and family, 36, 57, 72, and 78 samples could basically represent 82 samples to monitor the information of bacterial diversity at different classification levels. At the classification level of genus and OTU, the number of corresponding bacterial groups detected when the number of samples reached 82 was still increasing. These preliminary results show that the soil has a relatively high level of bacterial diversity in the evergreen and deciduous broad-leaved mixed forest, which lays the foundation for further studies on bacterial and plant diversity.





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