马俊杰1,2,
薛璐1,3,
田园1,
刘丹1
1. 西北大学, 西安 710127;
2. 二氧化碳捕集与封存技术国家地方联合工程研究中心, 西安 710127;
3. 榆林学院, 榆林 719000
作者简介: 赵晨阳(1994-),女,硕士研究生,研究方向为生态影响与生态监测研究,E-mail:ZCYsuk9898@163.com.
基金项目: 国家高技术研究发展计划(863计划)资助项目(2012AA0501030)；陕西省教育厅自然科学研究计划资助项目(19JK1001)中图分类号: X171.5
Toxic Effects of CO2 Leakage from Geological Storage on Earthworms
Zhao Chenyang1,Ma Junjie1,2,
Xue Lu1,3,
Tian Yuan1,
Liu Dan1
1. Northwest University, Xi'an 710127, China;
2. National & Local Joint Engineering Research Center of Carbon Capture and Storage Technology, Xi'an 710127, China;
3. Yulin University, Yulin 719000, China
CLC number: X171.5
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摘要:二氧化碳捕集与封存技术(CO2 capture and storage, CCS)是当前国际上公认的CO2减排的有效措施，但封存在地下的CO2仍然因为各种不稳定因素存在泄漏风险，对土壤环境及土壤生态系统产生威胁。选择赤子爱胜蚓为研究对象，通过模拟高浓度CO2对蚯蚓形态与生理变化的影响，探究CCS泄漏所产生的土壤高浓度CO2对蚯蚓的毒性效应。研究表明，土壤高浓度CO2使蚯蚓出现生殖环带肿大、尾部串珠以及断尾等外部形态变化，皮肤和刚毛受到损伤并且表皮发生褶皱等现象；随着CO2浓度的增加以及暴露时间的延长，蚯蚓的死亡率不断增加，土壤高浓度CO2对蚯蚓的7 d和14 d半致死浓度分别为26.39%和17.78%；蚯蚓体腔细胞溶酶体中性红保留时间(NRRT)减少。因此，蚯蚓有望作为监测CO2泄漏的指示生物，NRRT可作为识别CO2泄漏的敏感指标。
关键词: CO2泄漏/
蚯蚓/
皮肤损伤/
死亡率/
中性红保留时间
Abstract:CO2 capture and storage (CCS) is currently recognized as an effective measure to reduce carbon dioxide emissions in the world. However, the CO2 stored underground has the risk of leakage because of various unstable factors, posing a threat to the soil environment and soil ecosystem. In this study, Eisenia fetida was selected as the research object. By simulating the effects of high concentration of CO2 on the morphology and physiological changes of earthworms, the toxic effects of high concentration of CO2 on the earthworms caused by CCS leakage were explored. The results showed that the high concentration of CO2 in the soil caused earthworms to have external morphological changes such as clitellum swelling, tail beading and tail breaking; skin and setae were damaged and epidermis was wrinkled. With the increase of CO2 concentration and the extension of exposure time, the mortality rate of earthworms increased continuously. The median lethal concentrations of 7 d and 14 d for high concentration CO2 soil were 26.39% and 17.78%, respectively, and the lysosomal membrane neutral red retention time (NRRT) of granulocytes decreased. Therefore, earthworms are expected to be used as indicators of monitoring CO2 leakage, and NRRT can be used as a sensitive biomarker for CO2 leakage.
Key words:CO2 leakage/
earthworm/
skin injury/
mortality rate/
NRRT.
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