江博1,
闫宝亨1,
刘瑞强1,
周琴1,
李大彬2,
戴捷1,,
1. 长江大学化学与环境工程学院, 荆州 434023;
2. 湖北省生态环境厅荆州生态环境监测中心, 荆州 434000
作者简介: 汪冬芳(1996-),女,硕士研究生,研究方向为污水处理,E-mail:2630945434@qq.com.
通讯作者: 戴捷,cjdxdaijie@163.com
基金项目: 国家自然科学基金资助项目（21173026）；湖北省自然科学基金重点项目（2013CFA107）中图分类号: X171.5
Interaction between Er(Ⅲ) and Rape Mitochondria
Wang Dongfang1,Jiang Bo1,
Yan Baoheng1,
Liu Ruiqiang1,
Zhou Qin1,
Li Dabin2,
Dai Jie1,,
1. College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434023, China;
2. Jingzhou Ecological Environment Monitoring Center, Department of Ecology and Environment of Hubei Province, Jingzhou 434000, China
Corresponding author: Dai Jie,cjdxdaijie@163.com
CLC number: X171.5
-->
摘要
HTML全文
图
参考文献
相关文章
施引文献
资源附件
访问统计
摘要:通过光谱法和Clark氧电极法研究了重稀土离子铒离子（Er（Ⅲ））对油菜线粒体结构与功能的影响。实验结果表明，Er（Ⅲ）诱导油菜线粒体发生肿胀，其浓度为600 μmol·L-1时，油菜线粒体肿胀程度达到最大；Er（Ⅲ）还促进了线粒体内膜对H+/K+的渗透，降低了线粒体膜的流动性。这说明，Er（Ⅲ）可诱导线粒体发生膜渗透性转化（MPT），透射电镜（TEM）观察到500 μmol·L-1 Er（Ⅲ）作用下的线粒体外膜受损，进一步验证了MPT的发生。Clark氧电极实验结果表明，随着Er（Ⅲ）浓度逐渐增大，线粒体的呼吸好氧速率不断下降，说明Er（Ⅲ）诱导线粒体发生MPT的同时抑制了油菜线粒体的呼吸作用。
关键词: Er(Ⅲ)/
油菜线粒体/
渗透性转换/
光谱法
Abstract:In this research, the effects of heavy rare earth ions of erbium (Er(Ⅲ)) on the structure and function of rape mitochondria were studied by spectroscopy and Clark oxygen electrode method. The experimental results indicated that the Er(Ⅲ) induced the swollen morphology of rape mitochondria. The swollen degree reached the maximum when the ion concentration is up to 600 μmol·L-1. At the same time, the presence of Er(Ⅲ) enhanced the permeabilization of H+ and K+ on mitochondrial inner membrane and reduced the fluidity of mitochondrial membranes. These results indicated that Er(Ⅲ) could induce membrane permeability transformation (MPT) in rape mitochondria, and the damage of mitochondrial outer membrane under the concentration of 500 μmol·L-1 Er(Ⅲ) was observed via transmission electron microscope (TEM), which further verified the occurrence of MPT. Clark oxygen electrode experiment showed that with the concentration of Er(Ⅲ) increasing, the respiratory aerobic rate of mitochondria decreased continuously, which indicated that Er(Ⅲ) can induce MPT in rape mitochondria and inhibited its respiration at the same time.
Key words:Er(Ⅲ)/
rape mitochondria/
mitochondrial permeability transformation/
spectroscopy.
| Elbasan F, Ozfidan-Konakci C, Yildiztugay E, et al. Rare-earth element scandium improves stomatal regulation and enhances salt and drought stress tolerance by up-regulating antioxidant responses of Oryza sativa[J]. Plant Physiology and Biochemistry, 2020, 152:157-169 |
| Skovran E, Martinez-Gomez N C. Just add lanthanides[J]. Science, 2015, 348(6237):862-863 |
| 王振宇. 稀土元素镧、铈对山白兰幼苗生长的影响[D]. 南宁:广西大学, 2019:1 Wang Z Y. Effects of lanthanum and cerium on the growth of Paramichelia baillonii seedlings[D]. Nanning:Guangxi University, 2019:1(in Chinese) |
| 金姝兰, 黄益宗. 土壤中稀土元素的生态毒性研究进展[J]. 生态毒理学报, 2014, 9(2):213-223Jin S L, Huang Y Z. A review on ecological toxicity of rare earth elements in soil[J]. Asian Journal of Ecotoxicology, 2014, 9(2):213-223(in Chinese) |
| Ying S M, Häcker G. Apoptosis induced by direct triggering of mitochondrial apoptosis proceeds in the near-absence of some apoptotic markers[J]. Apoptosis, 2007, 12(11):2003-2011 |
| Zhao J, Jin J C, Zhou Z Q, et al. High concentration of gadolinium ion modifying isolated rice mitochondrial biogenesis[J]. Biological Trace Element Research, 2013, 156(1-3):308-315 |
| Dai J, Li C L, Zhang Y Z, et al. Effect of La3+ on heat production by mitochondria isolated from hybrid rice[J]. Thermochimica Acta, 2008, 470(1-2):77-82 |
| Li Q Q, Zhong H Q, Cao Y. Effective extraction and recovery of rare earth elements (REEs) in contaminated soils using a reusable biosurfactant[J]. Chemosphere, 2020, 256:127070 |
| 王巍敏, 李云昌, 胡琼, 等. 油菜高纯度线粒体DNA的高效提取及检测[J]. 中国油料作物学报, 2008, 30(3):357-360Wang W M, Li Y C, Hu Q, et al. An efficient protocol for pure mitochondrial DNA extraction from oilseed rape[J]. Chinese Journal of Oil Crop Sciences, 2008, 30(3):357-360(in Chinese) |
| 陈健美. 甘蓝型油菜2个细胞质雄性不育系的线粒体基因组研究[D]. 南京:南京农业大学, 2011:28-30 Chen J M. Studies on mitochondrial genomes of the two CMS lines in Brassica napus L.[D]. Nanjing:Nanjing Agricultural University, 2011:28-30(in Chinese) |
| Xia C F, Zhao J, Jin J C, et al. Ce(Ⅲ)-induced rice mitochondrial permeability transition investigated by spectroscopic and microscopic studies[J]. Biological Trace Element Research, 2013, 152(2):284-291 |
| Fernandes M A S, Custódio J B A, Santos M S, et al. Tetrandrine concentrations not affecting oxidative phosphorylation protect rat liver mitochondria from oxidative stress[J]. Mitochondrion, 2006, 6(4):176-185 |
| Ricchelli F, Dabbeni-Sala F, Petronilli V, et al. Species-specific modulation of the mitochondrial permeability transition by norbormide[J]. Biochimica et Biophysica Acta, 2005, 1708(2):178-186 |
| Borenstain V, Barenholz Y. Characterization of liposomes and other lipid assemblies by multiprobe fluorescence polarization[J]. Chemistry and Physics of Lipids, 1993, 64(1-3):117 |
| Zhang Y, Tian F F, Xiao Q, et al. Exploiting the role of resveratrol in rat mitochondrial permeability transition[J]. The Journal of Membrane Biology, 2013, 246(5):365-373 |
| Gzyl J, Przymusiński R, Gwóz'dz' E A. Ultrastructure analysis of cadmium-tolerant and -sensitive cell lines of cucumber (Cucumis sativus L.)[J]. Plant Cell, Tissue and Organ Culture, 2009, 99(2):227-232 |
| Salimi A, Bahreini F, Jamali Z, et al. Mesalazine induces oxidative stress and cytochrome c release in isolated rat heart mitochondria:An analysis of cardiotoxic effects[J]. International Journal of Toxicology, 2020, 39(3):241-247 |
| Yang L Y, Gao J L, Gao T, et al. Toxicity of polyhydroxylated fullerene to mitochondria[J]. Journal of Hazardous Materials, 2016, 301:119-126 |
| Dingeldein A P G, Sparrman T, Gröbner G. Oxidatively stressed mitochondria-mimicking membranes:A molecular insight into their organization during apoptosis[J]. Biochimica et Biophysica Acta Biomembranes, 2018, 1860(12):2644-2654 |
| Khandelwal N K, Sarkar P, Gaur N A, et al. Phosphatidylserine decarboxylase governs plasma membrane fluidity and impacts drug susceptibilities of Candida albicans cells[J]. Biochimica et Biophysica Acta Biomembranes, 2018, 1860(11):2308-2319 |
| Ma L, Dong J X, Fu W R, et al. Mitochondrial morphology and function impaired by dimethyl sulfoxide and dimethyl formamide[J]. Journal of Bioenergetics and Biomembranes, 2018, 50(4):297-305 |
| Gao J L, Wu M, Wang X, et al. Membrane permeability transition and dysfunction of rice mitochondria effected by Er(Ⅲ)[J]. The Journal of Membrane Biology, 2015, 248(1):39-46 |
