李香^2,3,
魏海峰^1,2,3,
刘长发^2,3,
宋雪^2,3,
赵肖依^2,3,
赵雨朦^2,3,
夏宁^2,3,
霍玉洁^2,3
1. 大连海洋大学, 农业农村部北方海水增养殖重点实验室, 大连 116023;
2. 大连海洋大学, 海洋科技与环境学院, 大连 116023;
3. 大连海洋大学, 近岸海洋环境科学与技术辽宁省高校重点实验室, 大连 116023

作者简介: 李香(2000-),女,河南省三门峡市人,本科,研究方向为海洋环境,E-mail:1332503139@qq.com.

基金项目: 辽宁省教育厅服务地方项目(No：DL201804)；国家海洋局溢油国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放基金(No：201309，201809)；国家海洋局近岸海域生态环境重点实验室基金(No：201013)；大连海洋大学农业农村部北方海水增养殖重点实验室基金(No：2018-KF-21)


中图分类号: X171.5

Effects of 2 Kinds of alkyl-PAHs on the Expression of CYP450 and p53 Genes of Apostichopus japonicus

Li Xiang^2,3,
Wei Haifeng^1,2,3,
Liu Changfa^2,3,
Song Xue^2,3,
Zhao Xiaoyi^2,3,
Zhao Yumeng^2,3,
Xia Ning^2,3,
Huo Yujie^2,3
1. Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China;
2. School of Marine Sciences and Environment, Dalian Ocean University, Dalian 116023, China;
3. Key Laboratory of Nearshore Marine Environmental Research of Liaoning Higher Education, Dalian Ocean University, Dalian 116023, China

CLC number: X171.5

-->

摘要
HTML全文
图(0)表(0)
参考文献(0)
相关文章
施引文献
资源附件(0)
访问统计

摘要:海洋环境中多环芳烃类(PAHs)主要来源于海洋溢油事故以及沿海石油化工企业的废水排放，国内外大量研究发现海洋中的多环芳烃对海洋生物造成了潜在的生态风险。为了揭示不同浓度多环芳烃类污染物对海参的生态毒理效应，将仿刺参(Apostichopus japonicus)分别暴露于不同浓度的2种烷基多环芳烃3-甲基菲(5、10、100 μg·L^-1)和2-甲基蒽(5、10、50 μg·L^-1)中，检测暴露3 d、7 d和14 d后，3-甲基菲和2-甲基蒽胁迫下仿刺参CYP450和p53基因的相对表达量。结果表明，3-甲基菲和2-甲基蒽胁迫下，仿刺参CYP450和p53基因的表达均对毒物产生了不同程度的响应。与对照组相比，3-甲基菲各处理组对仿刺参CYP450和p53基因的表达均产生显著的抑制作用(P<0.05)；2-甲基蒽各处理组对仿刺参CYP450和p53基因的表达影响作用不同，暴露7 d后，2-甲基蒽各处理组对仿刺参CYP450基因的表达表现出抑制作用，对p53基因的表达表现出诱导作用。相同浓度与时间胁迫下，2-甲基蒽对仿刺参CYP450和p53基因表达的影响比3-甲基菲的影响大。上述研究结果表明，3-甲基菲和2-甲基蒽均可不同程度影响仿刺参CYP450和p53基因的表达，且与3-甲基菲相比，2-甲基蒽对仿刺参CYP450和p53基因表达的影响较明显。上述结果为多环芳烃类污染物对仿刺参的生物毒性评价提供了基础数据。
关键词: 3-甲基菲/
2-甲基蒽/
仿刺参/
CYP450/
p53/
基因表达

Abstract:Marine organisms are facing a risk of exposure to a wide variety of toxic chemicals, such as polycyclic aromatic hydrocarbons (PAHs) from oil spill incidents and discharge of petrochemical wastewater. Besides, this kind of environmental pollutants pose a threat to the sea farming. As such, Apostichopus japonicus was exposed to 3-methylphenanthrene (5, 10 and 100 μg·L^-1) and 2-methylanthracene(5, 10 and 50 μg·L^-1) to study the toxic effects of alkyl-PAHs on the marine animals. Relative expression levels of CYP450 and p53 genes of Apostichopus japonicus under the stress of 3-methylphenanthrene and 2-methylanthracene were measured after 3 d, 7 d and 14 d. Results showed that 3-methylphenanthrene had significant inhibitory effect (P<0.05) on the expression of both CYP450 and p53 genes, while 2-methylanthracene had a different effect on the expression of CYP450 and p53 genes. From 7^th day onwards, CYP450 gene expression was inhibited by 2-methylanthracene, but p53 gene expression was induced. Meanwhile, Apostichopus japonicus were more sensitive to 2-methylanthracene than 3-methylphenanthrene within the same concentration range and exposure duration. These results indicated that 3-methylphenanthrene and 2-methylanthracene could affect the expression of CYP450 and p53 genes of Apostichopus japonicus, and compared with 3-methylphenanthrene, 2-methylanthracene has significant effect on the expression of CYP450 and p53 genes. These results provide the basic data for the biotoxicity evaluation of PAHs pollutants on Apostichopus japonicus.
Key words:3-methylphenanthrene/
2-methylanthracene/
Apostichopus japonicus/
CYP450/
p53/
gene expression.