薛士鹏¹,
王涵¹,
宋国英²,
华春秀¹,
于瑞雪²,
刘庆春¹,
王中晓¹,
张庆远¹,
刘丽²,
李冰洁²,
夏西超^1,2,,
1. 南阳医学高等专科学校基础医学部, 南阳 473061;
2. 平顶山学院医学院, 平顶山 476000

作者简介: 薛士鹏(1983-),男,硕士,研究方向为分子生物学,E-mail:409195235@qq.com.

通讯作者: 夏西超,xiaxichao8336@163.com

基金项目: 河南省高等学校重点科研项目(18A330004)；河南省联合基金项目(182300410123)；中国博士后基金项目(2016M590143)


中图分类号: Q459

Molecular Characterization of AwHSP70 from Freshwater Bivalve Anodonta woodiana: Effects of PBDE-47 on Its Expression

Xue Shipeng¹,
Wang Han¹,
Song Guoying²,
Hua Chunxiu¹,
Yu Ruixue²,
Liu Qingchun¹,
Wang Zhongxiao¹,
Zhang Qingyuan¹,
Liu Li²,
Li Bingjie²,
Xia Xichao^1,2,,
1. College of Basic Medicine, Nanyang Medical University, Nanyang 473061, China;
2. College of Medicine, Pingdingshan University, Pingdingshan 476000, China

Corresponding author: Xia Xichao,xiaxichao8336@163.com

CLC number: Q459

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摘要:热休克蛋白(HSP)参与蛋白质折叠、细胞膜转位和错误折叠蛋白质降解等过程，提高动物对环境的应激能力和适应能力。前期研究表明，多溴联苯醚-47(PBDE-47)对背角无齿蚌具有显著的氧化应激和急性毒性效应，为探讨PBDE-47慢性毒性效应；将背角无齿蚌随机分为对照组和PBDE-47处理组，处理组用3.36 μg·L^-1浓度的PBDE-47进行处理，对照组用相同体积的二甲亚砜进行处理；克隆出AwHSP70基因，分析PBDE-47对AwHSP70表达的影响。结果显示，AwHSP70具有HSP70家族的标签序列，广泛分布于斧足、鳃、肝胰脏、闭壳肌、心脏、血淋巴和外套膜。PBDE-47处理可导致肝胰脏、鳃和血细胞中AwHSP70 mRNA水平显著升高。与对照组相比，PBDE-47处理组肝胰脏中AwHSP70 mRNA水平在1~15 d内增加2.79倍(P＜0.01)以上；鳃中AwHSP70 mRNA水平增加3.06倍(P＜0.01)以上；血淋巴中AwHSP70表达增加1.81倍(P＜0.05)以上。背角无齿蚌上调AwHSP70表达有助于增强动物对PBDE-47的耐受能力。
关键词: PBDE-47/
背角无齿蚌/
AwHSP70

Abstract:Heat shock proteins (HSPs) play an important role in adaption to environmental stress by protein folding, membrane translocation, degradation of misfolded proteins and other regulatory processes. Our previous study showed oxidative stress generated from polybrominated diphenyl ether-47 (PBDE-47) could cause an acute toxicity to freshwater bivalve Anodonta woodiana, but the effect of chronic exposure need to be elucidated. In order to further investigate the chronic effect of PBDE-47, clams A. woodiana were randomly divided into the PBDE-47 treated group administrated with PBDE-47 at a concentration of 3.36 μg·L^-1 and control group treated with a similar volume dimethyl sulfoxide. One complete HSP sequence was isolated from A. woodiana and named AwHSP70. AwHSP70 was widely distributed in foot, gill, hepatopancreas, adductor muscle, heart, hemocytes and mantle. Administration of PBDE-47 could result in a significant up-regulation of AwHSP70 expression in the hepatopancreas, gill and hemocytes. In the hepatopancreas, mRNA level of AwHSP70 increased more than 2.79 times (P＜0.01) compared with that of control group. In the gill, during the experiment process, significant up-regulation of AwHSP70 expression showed a reversed U shape. In the hemocytes, AwHSP70 expression of PBDE-47 treated group increased more than 1.81 times (P＜0.05) compared with that of control group. These results indicated that up-regulation of AwHSP70 expression accounts for enhancing adaption of bivalve A. woodiana exposed to PBDE-47.
Key words:PBDE-47/
Anodonta woodiana/
AwHSP70.