张瑾^1,2,
姜慧¹,
董欣琦¹,
卞志强¹
1. 安徽建筑大学环境与能源工程学院, 安徽省水污染控制与废水资源化重点实验室, 合肥 230601;
2. 清华大学新兴有机污染物控制北京市重点实验室, 北京 100084

作者简介: 张瑾(1978-),女,博士后,副教授,硕士生导师,主要研究方向为生态毒理学,E-mail:ginnzy@163.com.

基金项目: 国家自然科学基金(No.21677001)；安徽省自然科学基金(No.1708085MB50)；安徽省重大科技专项(No.17030801028)


中图分类号: X592

Extended Isobologram for Predicting Antagonism between Pyridinium-based Ionic Liquids and Dodine

Zhang Jin^1,2,
Jiang Hui¹,
Dong Xinqi¹,
Bian Zhiqiang¹
1. Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China;
2. Beijing Key Laboratory for Emerging Organic Contaminants Control, Tsinghua University, Beijing 100084, China

CLC number: X592

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摘要:等效线图法(isobologram)是评估化学混合物毒性相互作用的经典方法之一，然而该方法仅能评估混合物在某一特殊浓度效应水平(通常为50%的浓度效应水平，即EC₅₀)的联合毒性作用情况。因此，拓展等效线图法并用于不同效应水平下混合物毒性的评估显得尤为必要。以杀菌剂多果定(Dod)和3种离子液体(ILs)包括溴化丁基吡啶([bpy]Br)、溴化己基吡啶([hpy]Br)和溴化辛基吡啶([opy]Br)为混合物组分，采用直线均分射线法设计3组二元混合物体系(Dod-[bpy]Br、Dod-[hpy]Br和Dod-[opy]Br)共15条射线，应用微板毒性分析法系统测定各污染物及其混合物射线对青海弧菌Q67(Vibro qinghaisiense sp. Q67, Q67)的毒性，应用拓展等效线图法分析15条混合物射线在5个不同效应水平(EC₂₀、EC₃₀、EC₄₀、EC₅₀和EC₆₀)的毒性相互作用，并与经典等效线图法和浓度加和模型(CA)评估的结果进行比较。结果表明：以pEC₅₀为毒性指标，3种吡啶ILs对Q67的毒性具有烷基链效应，即毒性大小顺序为Dod-[opy]Br>Dod-[hpy]Br>Dod-[bpy]Br；3组二元混合物体系的15条射线的毒性，随农药Dod浓度比的减少而减弱；拓展等效线图法可以比较直观地表征3组Dod-ILs混合物体系在5个不同效应水平的拮抗作用，且拮抗作用强度随Dod浓度比的增加而变化，即先增强后减弱；拓展等效线图法可以有效地评估二元混合物在多个效应水平的联合毒性相互作用。
关键词: 离子液体/
多果定/
Q67/
拮抗作用/
拓展等效线图法/
化学混合物

Abstract:Isobologram is a classical graphical analysis method for the evaluation of toxicity interaction within chemical mixtures. However, the method can only evaluate the toxicity interaction within mixtures at some certain concentration-effect level (such as EC₅₀). Therefore, it is necessary to extend the isobologram to evaluate mixture toxicity interaction at different effect levels. Three pyridinium-based ionic liquids (ILs), bromide butyl pyrimidine ([bpy]Br), bromide hexyl pyrimidine ([hpy]Br), bromide oxyl pyrimidine ([opy]Br) and a fungicide (Dodine, Dod), were selected as mixture components. Three binary mixture systems (Dod-[bpy]Br, Dod-[hpy]Br, Dod-[opy]Br) with 15 rays were designed by using the direct equipartition ray design procedure. The toxicity of single chemicals and their 15 binary mixture rays to Vibrio qinhaiensis sp.-Q67 were determined by the microplate toxicity analysis. The extended isobologram was used to analyze the joint toxicity interaction of the 15 rays at five different effect levels (EC₂₀, EC₃₀, EC₄₀, EC₅₀ and EC₆₀). The obtained results were compared to those predicted by the classical isobologram and concentration addition (CA) model. The results showed that the toxicity of three ILs to Q67 had alkyl-side chain effect according to the negative logarithm of median effective concentration (pEC₅₀). The toxicity of three binary mixture systems gradually reduced with concentration ratios of Dod in the mixture rays decreasing. The extended isobologram could well and clearly evaluate the toxicity interaction within three Dod-IL mixtures showing obviously antagonistic at 5 different effect levels. And the antagonistic effects firstly augmented and then weakened with the concentration ratio of Dod increasing. So the extended isobologram can effectively evaluate the joint toxicity interactions within binary mixtures.
Key words:ionic liquid/
dodine/
Q67/
antagonism/
extended isobologram/
chemical mixtures.