李如美¹,
戴争¹,
王维²,
张霞¹,
郇恒尚¹,
李丹丹¹,
高宗军^1,,
1. 山东省农业科学院植物保护研究所/山东省植物病毒学重点实验室, 济南 250100;
2. 南京南农农药科技发展有限公司, 南京 210095

作者简介: 李如美(1981-),女,助理研究员,研究方向为农药及昆虫毒理学,E-mail:rumeili0815@163.com.

通讯作者: 高宗军,gaozongjun@163.com

基金项目: 国家重点研发计划-化学农药协同增效技术及产品研发(2016YFD0200500)；山东省农业科学院农业科技创新工程(CXGC2016B11)


中图分类号: X171.5

Acute Toxicity and Bioconcentration of Metconazole in Brachydanio rerio

Li Rumei¹,
Dai Zheng¹,
Wang Wei²,
Zhang Xia¹,
Huan Hengshang¹,
Li Dandan¹,
Gao Zongjun^1,,
1. Institute of Plant Protection, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Plant Virology, Jinan 250100, China;
2. Nanjing Nannong Pesticide Technology Development Co. Ltd., Nanjing 210095, China

Corresponding author: Gao Zongjun,gaozongjun@163.com

CLC number: X171.5

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摘要:为评价杀菌剂叶菌唑对水生生态系统的影响，以斑马鱼(Brachydanio rerio)为试验生物，采用半静态法研究了叶菌唑原药对斑马鱼的急性毒性以及生物富集效应。结果表明，95%叶菌唑原药对斑马鱼96 h的半数致死浓度(96 h-LC₅₀)为3.89 mg·L^-1，选用在3.90×10^-1和3.90×10^-2 mg·L^-1这2个处理浓度下连续暴露8 d，在3.90×10^-1 mg·L^-1浓度下生物富集系数(BCF)为53.3；在3.90×10^-2 mg·L^-1浓度暴露下生物富集系数(BCF_8d)为26.2。根据《化学农药环境安全评价试验准则》毒性划分标准，叶菌唑原药属于中等富集性农药。研究结果为叶菌唑田间安全使用提供理论依据。
关键词: 叶菌唑/
斑马鱼/
急性毒性/
生物富集

Abstract:In order to evaluate the effect of metconazole on aquatic ecosystem, Brachydanio rerio was used as a tiral organism, the acute toxicity and bioenrichment effects of metconazole on Brachydanio rerio were studied through semi-static method. The results showed that the median lethal concentration (96 h-LC₅₀) of 95% metconazole for Brachydanio rerio was 3.89 mg·L^-1. When the continuous exposures were set at 3.90×10^-1 and 3.90×10^-2 mg·L^-1 metconazole for 8 days, the bioconcentrationt coefficient was 53.3 and 26.2, respectively. According to the Environmental Safety Evaluation Test Guideline of Chemical Pesticides, metconazole is a kind of medium bioconcentration pesticide. This study provides a theoretical basis for the safe use of metconazole in the field.
Key words:metconazole/
Brachydanio rerio/
acute toxicity/
bioaccumulation.