张莉¹,
韩娟²,
刘标¹
1. 环境保护部南京环境科学研究所, 环境保护生物安全重点实验室, 南京 210042;
2. 农业部食物与营养发展研究所, 北京 100081

作者简介: 张莉(1987-),女,甘肃白银人,研究方向为转基因生物安全,E-mail:feiniao8897@126.com.

基金项目: 转基因生物新品种培育重大专项(2016ZX08012-005)；环保公益性行业科研专项(201509044)


中图分类号: X171.5

Ecotoxicological Effects of Genetically Modified Maize C0030.3.5 with cry1Ab and epsps Gene on Daphnia magna

Zhang Li¹,
Han Juan²,
Liu Biao¹
1. Key Laboratory on Biosafety of National Environmental Protection, Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China;
2. Institute of Food and Nutrition Development, Ministry of Agriculture, Beijing 100081, China

CLC number: X171.5

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摘要:随着抗虫和耐草甘膦除草剂转基因玉米的迅速推广和种植，其环境安全性也越来越成为人们关注的焦点。为探讨抗虫耐除草剂转基因玉米C0030.3.5(外源基因cry1Ab和epsps)对水生动物环境的安全性，以模式生物大型蚤(Daphnia magna)为指示生物，分别使用1.5 g·L^-1 C0030.3.5玉米粉和其非转基因对照DBN318玉米粉饲喂大型蚤28 d，探讨C0030.3.5玉米对大型蚤生长和繁殖的影响。结果显示，C0030.3.5玉米粉组大型蚤与亲本DBN318组大型蚤相比，体长、存活率、新生幼蚤总数等没有显著性差异(P> 0.05)。28 d饲喂实验结果表明，抗虫耐除草剂玉米C0030.3.5没有对大型蚤生长和繁殖产生不良影响。上述研究结果为转基因玉米的商业化种植和安全管理提供科学数据。
关键词: 转基因玉米/
C0030.3.5/
大型蚤/
生态毒性/
饲喂试验

Abstract:Along with the rapid spread and plantation of maize that is resistant to insects and glyphosate, the environmental safety of genetically modified (GM) maize becomes the focus of attention. To discuss the environmental safety of genetically modified (GM) maize C0030.3.5 expressing the cry1Ab and EPSPS protein, water flea Daphnia magna, a crustacean arthropod commonly used as a model organism in ecotoxicological studies, were fed a basal diet of maize flours from C0030.3.5 or its parental maize DBN318 at the concentration of 1.5 g·L^-1 (per individual per day) in a 28-day study. No significant differences were observed between D. magna feeding C0030.3.5 and non-GM flour on survival, body size and reproduction. These results suggested GM maize C0030.3.5 showed negative effect on neither growth nor reproduction of D. magna and provided a scientific basis for the commercial application and environmental safety management of the transgenic maize in China.
Key words:genetically modified maize/
C0030.3.5/
Daphnia magna/
ecotoxicological effects/
feeding study.