赵情^1,2,3,
米铁柱^1,2,3,
甄毓^1,2,3,
石岩^1,2,3,
王国善^1,2,3
1. 中国海洋大学环境科学与工程学院, 青岛 266100;
2. 中国海洋大学海洋环境与生态教育部重点实验室, 青岛 266100;
3. 海洋国家实验室海洋生态与环境科学功能实验室, 青岛 266071

作者简介: 赵情(1990-),女,硕士研究生,研究方向为海洋生态学,E-mail:zhaoqouc2013@163.com.

基金项目: 青岛海洋科学与技术国家实验室鳌山科技创新计划项目“近海生态灾害发生机理与防控策略”(2016ASKJ02)；山东省自然科学基金项目(ZR2014DM007)；青岛市博士后人员应用研究项目“胶质类水母响应海洋低氧分子标记的筛选与应用”


中图分类号: X171.5

Norfloxacin Toxicity Effects on Gene Expression of Heat Shock 70 kDa Protein, GTP-binding Protein, and Oxidative Stress Protein of Aurelia aurita Polyps

Zhao Qing^1,2,3,
Mi Tiezhu^1,2,3,
Zhen Yu^1,2,3,
Shi Yan^1,2,3,
Wang Guoshan^1,2,3
1. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;
2. Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China;
3. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China

CLC number: X171.5

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摘要:诺氟沙星(norfloxacin, NFLX)广泛应用于水产养殖中的鱼类细菌性疾病治疗。为探讨诺氟沙星对海洋生物的毒性作用，选择海月水母螅状体为受试生物，考察了不同浓度诺氟沙星和不同暴露时间对GTP结合蛋白(GTP binding protein)、氧化应激蛋白(oxidative stress protein)和热休克70 kDa蛋白(heat shock 70 kDa protein, Hsp70)表达量的影响。结果表明，NFLX对海月水母螅状体的48 h的半致死浓度(48 h-LC₅₀)是415.1 mg·L^-1，NFLX对海月水母螅状体的毒性属于低毒。随着NFLX浓度的升高和培养时间的延长，Hsp70基因在第7天浓度300 mg·L^-1时表达量受到显著性诱导(P < 0.05)，较对照组升高9.1倍；GTP结合蛋白基因和氧化应激蛋白基因的表达量都呈现先急剧升高后降低的趋势。2个基因均在第1天受到显著性诱导(P < 0.05)，分别在NFLX浓度100 mg·L^-1和300 mg·L^-1时表达量达到最大值，较对照组升高10.15倍和50.5倍。Hsp70基因、GTP结合蛋白基因和氧化应激蛋白基因在实验期间都对NFLX表现出较好的响应。
关键词: 诺氟沙星/
海月水母螅状体/
Hsp70基因/
GTP结合蛋白基因/
氧化应激蛋白基因/
基因表达

Abstract:Norfloxacin is widely used in the bacterial disease treatments in aquaculture. In order to study the norfloxacin toxicity to marine organisms, Aurelia aurita polyps was exposed to NFLX to investigate its toxicity effects on the gene expression of GTP-binding protein, oxidative stress protein and heat shock 70 kDa protein. The results indicated LC₅₀ of NFLX at 48 h was 415.1 mg·L^-1, showing low toxicity to Aurelia aurita polyps. Hsp70 gene expression proliferated significantly with increasing time and concentration of NFLX, reaching the highest value at the seventh day at the NFLX concentration of 300 mg·L^-1, which was up to 9.1-fold higher than the control group (P < 0.05). The gene expression of GTP binding protein and oxidative stress protein both sharply increased at the initial stages and gradually decreased. And they were significantly induced at the first day (P < 0.05), reaching the highest value which were up to 10.15-fold and 50.5-fold higher than the control groups at the NFLX concentrations of 100 mg·L^-1 and 300 mg·L^-1, respectively. The genes of Hsp70, GTP binding protein and oxidative stress protein showed good responses to NFLX in the experimental period.
Key words:norfloxacin/
Aurelia aurita polyps/
heat shock 70 kDa protein gene/
GTP binding protein gene/
oxidative stress protein gene/
gene expression.