洪响声^1,2,
覃剑晖¹,
王子健²,
查金苗²
1. 华中农业大学水产学院, 武汉 430070;
2. 中国科学院生态环境研究中心 环境水质学国家重点实验室, 北京 100085

作者简介: 洪响声(1990-),男,硕士,研究方向为水生态毒理学,E-mail:Soundhong@163.com.

基金项目: 国家水体污染控制与治理科技重大专项(2014zx-07204-008-003)


中图分类号: X171.5

Cloning and Sequence Analysis of Genes Involved in Adult Neurogenesis of Rare Minnow (Gobiocypris rarus)

Hong Xiangsheng^1,2,
Qin Jianhui¹,
Wang Zijian²,
Zha Jinmiao²
1. College of Fisheries, Huazhong Agriculture University, Wuhan 430070, China;
2. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China

CLC number: X171.5

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摘要:成体神经发生是脊椎动物中广泛存在的一种生物学特征。成体硬骨鱼类的脑展现出强烈的神经活性以及出色的脑修复能力，这使得硬骨鱼类成为研究成体神经发生和脑修复的一个理想的模型。本文克隆了成体稀有鮈鲫(Gobiocypris rarus)脑组织中神经发生及脑修复相关的hes5、pax6、sox11和prox1基因的部分cDNA序列并进行了序列分析。序列分析结果表明，pax6和sox11基因片段与斑马鱼(Danio rerio)对应的基因片段的同源性最高，分别为97%和94%； hes5基因与鲤鱼(Cyprinus carpio)相对应的基因片段的同源性最高，为92%；prox1基因在物种间的同源性最低。基于稀有鮈鲫和已知物种相应基因的核苷酸序列构建了系统发育树，发现稀有鮈鲫prox1基因与其他硬骨鱼类的亲缘关系最远。本文为进一步开展神经毒性类化学品对水生生物鱼类的成体神经毒性作用机制研究提供了分子生物学基础。
关键词: 稀有鮈鲫/
神经发生/
基因克隆/
同源性分析/
系统发育树

Abstract:Adult neurogenesis is a widespread trait of vertebrates. The brain of the adult teleost fish exhibits intense neurogenic activity and outstanding capability for brain repair, which makes teleost fish a ideal model for the study of adult neurogenesis and brain regeneration. In this paper, we cloned the partial cDNA sequence of hes5, pax6, sox11, and prox1 from the brain of Gobiocypris rarus, which are involved in neurogenesis and brain regeneration, and analyzed the sequences. Sequence analysis showed that pax6 and sox11 genes shared the highest sequence affinity with genes of Danio rerio(97% and 94%, respectively); hes5 gene shared the highest sequence affinity with the gene of Cyprinus carpio (92%); prox1 gene shared the lowest sequence affinity among species. The phylogenetic trees were developed based on the nucleotide sequences of the corresponding genes of known species, which showed the fartherest phylogenetic relationship of rare minnow prox1 gene and other teleosts. In this paper, we provide a basic molecular biological basis for the further research on the mechanism of neurotoxicity of aquatic organisms.
Key words:Gobiocypris rarus/
neurogenesis/
clone/
homology analysis/
phylogenetic trees.