司万童^1,2,3,
蒋海明³,
栗利曼³,
刘莎³,
刘菊梅³,
沈渭寿²,
朱晓东¹
1. 南京大学环境学院污染控制与资源化利用国家重点实验室, 南京 210023;
2. 环境保护部南京环境科学研究所, 南京 210042;
3. 内蒙古科技大学生命科学与技术学院, 包头 014010

作者简介: 司万童(1986-),男,副教授,研究方向为生态毒理学和环境生物学,E-mail:siwt02@163.com.

基金项目: 国家科技基础性工作专项(2014FY110800)；国家自然科学基金(31460142)；中国博士后科学基金面上项目(2015M571663)；内蒙古科技大学创新基金青年学术骨干培养专项(2015QNGG05)


中图分类号: X171.5

The Response of Antioxidant Enzyme in Bufo raddei Gonad to Rare Earth Tailings Pollution Stress

Si Wantong^1,2,3,
Jiang Haiming³,
Li Liman³,
Liu Sha³,
Liu Jumei³,
Shen Weishou²,
Zhu Xiaodong¹
1. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210042, China;
2. Nanjing Institute of Environmental Science, Ministry of Environmental Protection, Nanjing 210042, China;
3. School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China

CLC number: X171.5

-->

摘要
HTML全文
图(0)表(0)
参考文献(0)
相关文章
施引文献
资源附件(0)
访问统计

摘要:本研究以花背蟾蜍(Bufo raddei)作为实验动物，以某稀土尾矿库周边受污染的水域湿地为研究样地，以相对无污染的小白河黄河湿地自然保护区为对照样地。通过检测两地花背蟾蜍精巢和卵巢的脏器系数、MDA和GSH含量、SOD、GSH-Px、GST、CAT和GR活性、T-AOC等相关指标。研究花背蟾蜍性腺抗氧化酶对该稀土尾矿库污染胁迫的响应效应。结果显示：与黄河湿地相比，稀土尾矿库湿地复合污染对花背蟾蜍精巢和卵巢脏器系数的影响差异不显著。稀土尾矿库花背蟾蜍精巢和卵巢中MDA含量、GPx、GST和CAT活性显著高于黄河湿地(P＜0.05)，而GSH含量表现出相反的趋势。精巢中T-AOC显著高于卵巢(P<0.01)，但相同组织在两地间差异不显著。结果表明该稀土尾矿库复合污染对花背蟾蜍精巢和卵巢抗氧化能力有一定的毒害效应，且精巢和卵巢对污染胁迫的响应有明显的差异性。在应对稀土尾矿库湿地复合污染胁迫时，精巢更倾向于调用SOD-CAT系统的抗氧化机制，而在卵巢内GSH系统发挥作用更大。
关键词: 尾矿库/
花背蟾蜍/
精巢/
卵巢/
脏器系数/
抗氧化/
毒性效应

Abstract:This study uses Bufo raddei as the experimental animal. The polluted wetland at south side of a rare earth tailing was chosen as the study site, and the Yellow River’s Xiaobaihe wetland as the control site. The indicators including organ coefficients, MDA and GSH contents, and SOD, GSH-Px, GST, CAT and GR activities, and T-AOC were measured to study the response of antioxidant system in Bufo raddei gonad to rare tailings pollution stress. The results showed that the testis and ovary organ coefficients of B. raddei in rare earth tailings site had no obvious difference compared with that in the Yellow River site. The MDA contents, and GPx, GST and CAT activities in testis and ovary of B. raddei in rare earth tailings site were significantly higher than that in the Yellow River site (P<0.01), while the GSH contents were lower than that in the Yellow River site. In both tailings and the Yellow River sites, T-AOC in the testis were significantly higher than that in the ovary (P＜0.05), but there was no obvious difference between two sites in the same organ. The results indicated that the pollution of this rare earth tailing had certain toxic effects on antioxidant capacities in the testis and ovary of B. raddei. The testis’ and ovary’s antioxidant enzymes showed different responses to the rare tailings pollution stress. The SOD/CAT antioxidant system contributed more to the tolerance to the stress in the testis, while the GSH-related antioxidant enzymes play a more important role in the ovary of B. raddei.
Key words:tailings/
Bufo raddei/
testis/
ovary/
organ coefficient/
antioxidant/
toxic effect.