张红霞,
崔瑞娜,
戴家银^,
中国科学院动物研究所 中国科学院动物生态与保护生物学重点实验室, 北京 100101

作者简介: 张红霞(1981-),女,助理研究员,研究方向为生态毒理学,E-mail:zhanghx@ioz.ac.cn.

通讯作者: 戴家银,daijy@ioz.ac.cn

基金项目: 国家自然科学基金(31320103915
21377128)


中图分类号: X171.5

Chronic Exposure of PFDoA Affect the Phosphorylation Levels of Proteins in Rat Liver

Zhang Hongxia,
Cui Ruina,
Dai Jiayin^,
Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Corresponding author: Dai Jiayin,daijy@ioz.ac.cn

CLC number: X171.5

-->

摘要
HTML全文
图(0)表(0)
参考文献(0)
相关文章
施引文献
资源附件(0)
访问统计

摘要:全氟烷基类化合物(PFASs)是一类新型持久性有机污染物，因其独特的理化性质被广泛应用于工业和商业领域，对生物体具有一定的毒性作用。为探究全氟十二烷酸(PFDoA)致肝脏毒性作用机制，选择雄性大鼠为受试生物，采用2-DE蛋白质组学技术与ProQ Diamond dye磷酸化蛋白染色结合的方法初步研究了不同剂量PFDoA暴露110 d后大鼠肝脏蛋白磷酸化水平的变化。结果表明，30个磷酸化蛋白表达水平在PFDoA处理后发生显著变化，其中，经过MALDI-TOF/TOF质谱分析，成功鉴定18个蛋白点。经过生物信息学分析，发现这些蛋白主要涉及糖脂代谢、氨基酸代谢、应激防御及电子传递等途径。以上结果有助于进一步从翻译后修饰水平了解PFDoA的肝脏毒性作用。
关键词: PFDoA/
大鼠/
肝脏毒性/
磷酸化蛋白/
Pro-Q Diamond

Abstract:Perfluoroalkyl and polyfluoroalkyl substances (PFASs), an emerging kind of persistent organic pollutant, have been widely used in commercial and industrial products due to their unique physicochemical properties. PFASs have various toxicities to the living system. Perfluorododecanoic acid (PFDoA) with twelve carbon atoms, has broad industrial applications and is widely distributed in both wildlife and the environment. However, unlike other PFASs with short carbon chains, up to date limited studies have been performed on the toxic effects of PFDoA on animals. To reveal the mechanism of PFDoA hepatotoxicity, male rats were exposed to PFDoA for 110 days and the phosphoprotein profiles in the rat liver were analyzed by 2-DE methods coupled with Pro-Q Dimond phosphoprotein stain (Pro-Q DPS). The results showed significant change in the phosphorylation levels of 30 proteins after PFDoA exposure. Among them, 18 proteins were identified successfully by MALDI-TOF/TOF MS analysis. After bioinformatics analysis, these identified phosphoproteins were found to be involved in lipid metabolism, glucose metabolism, stress and immune response, electron transport, and so on. The results presented in this study will provide basic data for further study of the mechanism of PFDoA hepatotoxicity at the post-translational modification level.
Key words:PFDoA/
rat/
hepatotoxicity/
phosphoprotein/
Pro-Q Diamond.