金长文
邮箱:changwen@pku.edu.cn北京大学长江特聘教授
传真:+86-10-62753790联系电话:+86-10-62756004海淀区颐和园路5号北京大学北京核磁共振中心,北京市,中国,邮政编码:100871

个人简历
科研领域描述主要研究方向：结构生物学，生物大分子动力学的核磁共振研究，生物核磁共振方法学。


Ourresearchmainlyfocusesonstudiesofthestructures,dynamicsandbiologicalfunctionsofproteins,especiallyenzymes.Inparticular,weareinterestedinthecorrelationbetweenproteindynamics(structuralfluctuationsovertime)andtheirfunctionalmechanisms.WeemployNuclearMagneticResonance(NMR)techniquetodeterminethesolutionstructuresandprobethedynamicpropertiesofbio-macromolecules.Wealsousebiochemicalmethodstocharacterizetheactivityandfunctionsofproteinsbothinvitroandinvivo.Ourmaingoalistoelucidatethestructuralanddynamicbasisofproteinfunctions.


Proteinstructuresanddynamics
Toprovidestructuralbasisfortheirbiologicalfunctions,weuseNMRtechniquetodeterminethesolutionstructuresofproteinsandproteincomplexes.However,proteinfunctionsessentiallydependontheirmotions,andstaticstructuresalonecannotfullyexplaintheirfunctions.NMRisapowerfultooltoprobethemotionalpropertiesofproteins.Therefore,weuserelaxationphenomena,aswellasnewlydevelopedNMRmethodssuchasPREandRDCtoinvestigateproteindynamicsandtheircorrelationtofunctions.



Proteininteractions
Proteinfunctionsthroughinteractionswithothermolecules(proteins,ligands,ornucleicacids).WeuseNMRmethodstocharacterizeproteininteractions.Inparticular,weareinterestedinstudying(1)interactionsbetweenenzymesandsubstratesduringcatalysis,(2)interactionsbetweenreceptorsandligandsinsignaltransductionpathways,(3)protein-proteininteractionsintheregulationofproteasome.



Membraneproteins
Membraneproteinsplayessentialrolesincells.RecentadvancesinbiochemicalandNMRtechniqueshavemadeitpossibletousesolutionNMRmethodtostudythestructuresanddynamicsofmembraneproteins.Weareinterestedinstudyingthestructuresofproteincross-membranetranslocationmachineries,andtoelucidatethemolecularmechanismorthetranslocationprocesses.


LabHomepage:http://bnmrc.pku.edu.cn/JinLab.html

代表性论文1.YunfeiHu,XinxinZhang,YunmingShi,YanfengZhou,WeiZhang,Xiao-DongSu,BinXia,JindongZhao*andChangwenJin*,StructuresofAnabaenacalcium-bindingproteinCcbP:insightintoCa2+signalingduringheterocystdifferentiation,J.Biol.Chem.,2011,286,12381
2.YunfeiHu,EnweiZhao,HongweiLi,BinXiaandW.J.ZhuandChangwenJin*,SolutionNMRstructureoftheTatAcomponentofthetwin-arginineproteintransportsystemfromGram-positivebacteriumBacillussubtilis,J.Am.Chem.Soc,2010,132,15942
3.EwenLescop,ZhenweiLu,QinLiu,HuiminXu,GuangyuLi,BinXia,HonggaoYanandChangwenJin*,DynamicsoftheconformationaltransitionsintheassemblingoftheMichaeliscomplexofabisubstrateenzyme:a15NrelaxationstudyofEscherichiacoli6-hydroxymethyl-7,8-dihydropterinpyrophosphokinase,Biochemistry48,2009,302
4.HongweiLi,FanYang,XueKang,BinXiaandChangwenJin*,SolutionstructuresandbackbonedynamicsofEscherichiacolirhodanesePspEinitssulfur-freeandpersulfide-intermediateforms:implicationsforthecatalyticmechanismofrhodanese,,Biochemistry47,2008,4377
5.FanYang,WeiHu,HuiminXu,CongminLi,BinXiaandChangwenJin*,SolutionstructureandbackbonedynamicsofanendopeptidaseHycIfromEscherichiacoli:implicationsformechanismofthe[NiFe]hydrogenasematuration,J.Biol.Chem.,2007,282,3856
6.YouLi,YunfeiHu,XinxinZhang,HuiminXu,EwenLescop,BinXiaandChangwenJin*,Conformationalfluctuationscoupledtothethiol-disulfidetransferbetweenthioredoxinandarsenatereductaseinBacillussubtilis,J.Biol.Chem.,2007,282,11078
7.XinxinZhang,YunfeiHu,XianrongGuo,EwenLescop,YouLi,BinXiaandChangwenJin*,TheBacillussubtilisYKUVisathiol-disulfideoxidoreductaserevealedbyitsredoxstructuresandactivity,J.Biol.Chem.,2006,281,8296
8.XueKang,YunfeiHu,YouLi,XianrongGuo,XiaoluJiang,LuhuaLai,BinXiaandChangwenJin*,Structural,biochemicalanddynamiccharacterizationsofthehRPB8subunitofhumanRNApolymerases,J.Biol.Chem.,2006,281,18216
9.YunfeiHu,YouLi,XinxinZhang,XianrongGuo,BinXiaandChangwenJin*,SolutionstructuresandbackbonedynamicsofaflavodoxinMioCfromEscherichiacoliinbothapo-andholo-forms:implicationsforcofactorbindingandelectrontransfer,J.Biol.Chem.,2006,281,35454
10.XianrongGuo,YouLi,KuanPeng,YunfeiHu,CongminLi,BinXiaandChangwenJin*,SolutionstructuresandbackbonedynamicsofarsenatereductasefromBacillussubtilis:reversibleconformationalswitchassociatedwitharsenatereduction,J.Biol.Chem,2005,280,39601