伊成器
邮箱:chengqi.yi@pku.edu.cn联系电话:+86-10-62752895北京市海淀区颐和园路5号,北京,中国,邮政编码:100871

个人简历
科研领域描述主要通过化学生物学、结构生物学、高通量测序等手段，对核酸修饰的生物功能及其生理调控机制进行研究。一方面，核酸的化学修饰对许多重要生命过程（比如5-甲基胞嘧啶及其氧化产物对基因表达及细胞发育过程、RNA编辑对选择性剪接及蛋白质翻译过程等等）都有着广泛而深远的影响；另一方面，异常的核酸修饰（比如DNA及RNA损伤）则会导致细胞衰老、死亡及致癌性变异等严重后果。因此，我们将通过灵敏、高效的检测手段的建立，对许多核酸修饰的产生及调控过程进行研究，并对其生物功能进行阐述。具体的研究内容包括：（1）新颖RNA修饰对小分子RNA（microRNA）及长链非编码RNA（lincRNA）功能的影响及机理；（2）碱基切除及核苷酸切除DNA修复通路在防止细胞衰老及癌变过程中的分子机制；（3）以核酸修饰为机理的表观遗传学。



Chemicalmodificationsofnucleicacidhavebroadandprofoundimpacttoalotofcentralprocessesoflife.Forexample,inDNA,5-methylcytosineanditsoxidizedproductsarefamousepigeneticmarkers;inRNA,adenosine-to-inosineeditinginfluencesalternativesplicingandtheproductsoftranslation,addinganotherlayerofcomplexitytogeneregulation.However,aberrantchemicalmodifications(i.e.DNAandRNAdamage)canresultinsenescence,celldeathandcanceraswell.Therefore,investigationsofthebiogenesis/formationandregulatory/cleaning-upmechanismofsuchmodificationsarehighlyurgednotonlyfortheinterestsoffundamentalsciencebutalsoforthepurposeofhealthandlife.

Wecombinechemicalbiologymethodswithstructuralbiology,cellbiology,high-throughputsequencingandchemicalsynthesisinordertostudymodificationsofinterest.Specifically,wefocusonthreetopics:(1)theimpactofnovelRNAmodificationstothebiologicalfunctionsofmicroRNAandlargeintergenicnon-codingRNA(lincRNA);(2)base-excisionrepairandnucleotideexcisionrepair:theirmechanismsandrolesinpreventingtumorgrowthandcancermetathesis;(3)nucleicacidmodification-mediatedepigeneticprocesses.

代表性论文1.ParisienM*,YiC*,PanT,Rationalizationandpredictionofselectivedecodingofpseudouridine-modifiednonsenseandsensecodons,RNA,2012,18(3):355-67,[FullText],(*:contributeequally)
2.YiC,PanT,CellulardynamicsofRNAmodification,AccountsofChemicalResearch,2011,20;44(12):1380-8,[FullText]
3.JiaG,FuY,ZhaoX,DaiQ,ZhengG,YangY,YiC,LindahlT,PanT,YangYG,HeC,N6-methyladenosineinnuclearRNAisamajorsubstrateoftheobesity-associatedFTO,NatureChemicalBiology,2011,16;7(12):885-7,[FullText]
4.SongCX,SzulwachKE,FuY,DaiQ,YiC,LiX,LiY,ChenCH,ZhangW,JianX,WangJ,ZhangL,LooneyTJ,ZhangB,GodleyLA,HicksLM,LahnBT,JinP,HeC,Selectivechemicallabelingrevealsthegenome-widedistributionof5-hydroxymethylcytosine,NatureBiotechnology,2011,29(1):68-72,[FullText]
5.YiC,JiaG,HouG,DaiQ,ZhangW,ZhengG,JianX,YangCG,CuiQ,HeC,Iron-catalysedoxidationintermediatescapturedinaDNArepairdioxygenase,Nature,2010,468(7321):330-3,[FullText]
6.YiC,YangCG,HeC,Anon-hemeiron-mediatedchemicaldemethylationinDNAandRNA,AccountsofChemicalResearch,2009,42(4):519-29,[FullText]
7.YangCG*,YiC*,DuguidEM,SullivanCT,JianX,RicePA,HeC,CrystalstructuresofDNA/RNArepairenzymesAlkBandABH2boundtodsDNA,Nature,2008,452(7190):961-5,[FullText],(*:contributeequally)