1. 福建师范大学生命科学学院,福建 福州 350117;2. 福建师范大学工业微生物教育部工程研究中心,福建 福州 350117; 3. 福清市新大泽螺旋藻有限公司,福建 福清 350300
收稿日期:
2017-09-01修回日期:
2017-10-24出版日期:
2018-06-22发布日期:
2018-06-06通讯作者:
陈必链基金资助:
“十三五”海洋经济创新发展示范项目子课题High Cell Density Fermentation of Chlorella Based on Kinetics Model
Youcai ZHOU1, Yongjin HE1,2, Linsheng LI3, Mingzi WANG1,2, Bilian CHEN1,2*, Xing ZHENG31. School of Life Science, Fujian Normal University, Fuzhou, Fujian 350117, China; 2. Engineering Research Center of Industrial Microbiology, Ministry of Education, Fuzhou, Fujian 350117, China; 3. Fuqing King Dnarmsa Spriulina Co., Ltd., Fuqing, Fujian 350300, China
Received:
2017-09-01Revised:
2017-10-24Online:
2018-06-22Published:
2018-06-06摘要/Abstract
摘要: 构建了50 L发酵罐小球藻分批培养动力学模型,采用补料策略高密度发酵培养小球藻,考察了补料发酵过程中碳源的利用情况,采用实时荧光定量PCR技术分析了蛋白质合成关键酶二氨基庚二酸异构酶(dapF)、柠檬酸合成酶(CS)和葡萄糖?6-磷酸脱氢酶(G6PDH)的基因表达情况. 结果表明,小球藻经补料培养120 h,细胞生物量达106.65 g/L,平均生长速率为0.89 g/(L?h),葡萄糖的细胞得率为0.56 g/g,发酵过程中葡萄糖和尿素浓度对小球藻的dspF, CS和G6PDH基因表达量有重要影响.
引用本文
周有彩 何勇锦 李林声 王明兹 陈必链 郑行. 基于发酵动力学模型的小球藻高密度发酵培养[J]. 过程工程学报, 2018, 18(3): 624-631.
Youcai ZHOU Yongjin HE Linsheng LI Mingzi WANG Bilian CHEN Xing ZHENG. High Cell Density Fermentation of Chlorella Based on Kinetics Model[J]. Chin. J. Process Eng., 2018, 18(3): 624-631.
使用本文
导出引用管理器 EndNote|Ris|BibTeX
链接本文:http://www.jproeng.com/CN/10.12034/j.issn.1009-606X.217318
http://www.jproeng.com/CN/Y2018/V18/I3/624
参考文献
[1]Krienitz L, Huss VA, Bock C.Chlorella: 125 years of the green survivalist[J][J].Trends Plant Sci, 2015, 20(2): 67-69 [2]Morales‐Sánchez D, Martinez‐Rodriguez OA, Martinez A.Heterotrophic cultivation of microalgae: production of metabolites of commercial interest[J][J].Journal of Chemical Technology & Biotechnology, 2017, 92(5):925-936 [3]李雄, 王伟良, 黄建科.微藻规模化培养技术研究进展及产业化概况[J][J].生物产业技术, 2016, (3):7-13 [4]LI X, Wang W L, Huang J K.Research progress of the microalgae scale-cultivation and the industrialization[J]. Biotechnology & Business. 2016, (3): 7-13. [5]Ogbonna JC, Moheimani NR.Potentials of Exploiting Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae[M]. Biomass and Biofuels from Microalgae, 2015, 2: 45-61. [6]Wang J, Yang H, Wang F.Mixotrophic cultivation of microalgae for biodiesel production: status and prospects[J]. Appl Biochem Biotechnol. 2014, 172(7): 3307-3329. [7]Ogbonna JC, McHenry MP.Culture Systems Incorporating Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae [M].Biomass and Biofuels from Microalgae 2015, 2: 63-74. [8]Palabhanvi B, Muthuraj M, Mukherjee M, et al.Process engineering strategy for high cell density-lipid rich cultivation of Chlorella sp. FC2 IITG via model guided feeding recipe and substrate driven pH control[J]. Algal Research. 2016, 16: 317-329. [9]Zheng Y, Li T, Yu X, et al.High-density fed-batch culture of a thermotolerant microalga Chlorella sorokiniana for biofuel production[J]. Applied Energy. 2013, 108: 281-287. [10]Singhasuwan S, Choorit W, Sirisansaneeyakul S, et al.Carbon-to-nitrogen ratio affects the biomass composition and the fatty acid profile of heterotrophically grown Chlorella sp. TISTR 8990 for biodiesel production[J]. J Biotechnol. 2015, 216: 169-177. [11]何勇锦, 柯汉伟, 陈兰孙, 等.粘红酵母RG发酵产油脂动力学模型的构建[J]. 福建师范大学学报:自然科学版. 2014, 30(3): 93-99 [12]He Y J, Ke H W, Chen L S, et al.Studies on the Fermentation Kinetics Models for the Lipid Production by Rhodotorula glutinis RG[J]. Journal of Fujian Normal University. 2014, 30(3): 93-99 [13]Sachdeva N, Kumar GD, Gupta RP, et al.Kinetic modeling of growth and lipid body induction in Chlorella pyrenoidosa under heterotrophic conditions[J]. Bioresour Technol. 2016, 218: 934-943. [14]Wu ZY, Shi CL, Shi XM.Modeling of lutein production by heterotrophic Chlorella in batch and fed-batch cultures[J]. World Journal of Microbiology and Biotechnology. 2007, 23(9): 1233-1238. [15]周德庆.微生物学教程-第2版[M]. 高等教育出版社, 2002. [16]Zhou D Q.A tutorial in microbiology. Second edition[M]. Higher Education Press, 2002. [17]徐一兰, 官春云, 谭太龙, 等.油菜种子形成中含油量与其合成相关酶活性的变化及其相关性[J]. 作物学报. 2008, 34(10): 1854-1857. [18]Xu Y L, Guan C Y, Tan T L, et al.Changes of Oil Content and Oil Biosynthesis-Related Enzymes Activities and Their Correlation during Seed Formation in Brassica napus[J]. Acta Agronomica Sinica |. 2008, 34(10):1854-1857. [19]王滕旭, 李正国, 杨迎伍, 等.甜橙柠檬酸合酶基因的克隆及其表达分析[J]. 中国农学通报. 2010, 26(10):65-69. [20]Wang T X, Li Z G, Yang Y W, et al.Cloning and Expression Analysis of Citrate Synthase Gene in Orange[J]. Chinese Agricultural Science Bulletin. 2010, 26(10): 65-69. [21]Ning H, Zhang Y, Ling H, et al.Cloning and Expression Analysis of a Diaminopimelate Epimerase Gene in Sugarcane[J]. Chinese Journal of Tropical Crops. 2013, 34(11): 2200-2208. [22]李兴武, 李元广, 沈国敏, 等.普通小球藻异养-光自养串联培养的培养基[J]. 过程工程学报. 2006, 6(2): 277-280. [23]Li X W, Li Y G, Shen G M, et al.Medium for Culturing Chlorella vulgaris with Sequential Heterotrophic?Autotrophic Model[J]. The Chinese Journal of Process Engineering. 2006, 6(2): 277-280. [24]任婷月, 周万里, 张利群, 等.一种检测葡萄糖氧化酶活力的新方法[J]. 食品与发酵工业. 2015, 41(1): 212-215. [25]Ren T Y, Zhou W L, Zhang L Q, et al.The new technology for detecting glucose oxidase activity[J]. Food and Fermentation Industries2015, 41(1): 212-215. [26]GB 5009.5-2010, 《食品中蛋白质的测定》. [27]GB 5009.5-2010, National food safety standard Determination of protein in foods. [28]Liang S, Zhu M, Meng H, et al.Heterotrophic mass cultures of Chlorella vulgaris with glucose feeding in fermenters[J]. Journal of South China University of Technology. 2000. 28(12): 66-70 [29]Fan J, Cui Y, Wan M, et al.Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosa under three nutrition stressors[J]. Biotechnol Biofuels. 2014, 7(1): 17. [30]Keating KA, Cherry S.Use and Interpretation of Logistic Regression in Habitat-Selection Studies[J]. Journal of Wildlife Management. 2004, 68(4): 774-789. [31]Luedeking R, Piret EL.A Kinetic Study of the Lactic Acid Fermentation[J]. Journal of Biochemical & Microbiological Technology & Engineering. 2000, 1(4): 393-412. [32]Xie T, Xia Y, Zeng Y, et al.Nitrate concentration-shift cultivation to enhance protein content of heterotrophic microalga Chlorella vulgaris: Over-compensation strategy[J]. Bioresour Technol. 2017. 233: 247-255 [33]Chia MA, Lombardi AT, Da GGMM, et al.Combined nitrogen limitation and cadmium stress stimulate total carbohydrates, lipids, protein and amino acid accumulation in Chlorella vulgaris (Trebouxiophyceae)[J]. Aquatic Toxicology. 2015, 160: 87. [34]丁小云, 诸葛斌, 方慧英, 等.产甘油假丝酵母补料发酵中的甘油合成衰减[J]. 应用与环境生物学报.2012, 18(5): 791-796. [35]Ding X Y, Zhuge B, Fnag H Y, et al.Glycerol Synthesis Attenuation of Candida glycerinogenes in Fed-batch Fermentation [J].Chinese Journal of Applied and Environmental Biology .2012, 18(5): 791-796. [36]孙启星, 陈旭升, 任喜东, 等.基于pH调节和有机氮源流加调控补料分批发酵过程提高ε-聚赖氨酸产量[J][J].生物工程学报, 2015, 31(5):752-756 [37]. [38]Ren X, Chen J, Deschênes JS, et al.Glucose feeding recalibrates carbon flux distribution and favours lipid accumulation in Chlorella protothecoides through cell energetic management[J].[J].Algal Research, 2016, 14:83-91 |
相关文章 15
[1] | 王艺璇 颜成虎 丛威. 塑料薄膜材料在微藻培养环境中的稳定性和生物附着行为[J]. 过程工程学报, 2020, 20(1): 74-83. |
[2] | 石贤爱 林海虹 王晓萍 方世银. 醇脱氢酶同工酶基因表达与酿酒酵母催化2-辛酮不对称还原的关系[J]. 过程工程学报, 2017, 17(4): 827-833. |
[3] | 胡嘉欢 张谷涵 付永前 朱婷婷. 米根霉一步发酵法高效积累L-乳酸的策略[J]. 过程工程学报, 2017, 17(2): 375-381. |
[4] | 黄建科康少锋李元广李伟. 蛋白核小球藻的光衰减模型及藻细胞受光特性参数的模拟[J]. , 2014, 14(2): 304-311. |
[5] | 吴夏芫宋天顺支银芳周楚新俞俊杰朱隽瑶. 小球藻生物阴极型微生物燃料电池的基础特性[J]. , 2012, 12(1): 131-135. |
[6] | 王航董清风孟春石贤爱郭养浩. 酿酒酵母转化生成2-苯乙醇分批补料工艺优化[J]. , 2010, 10(4): 767-771. |
[7] | 何辉冯雅丽李浩然李顶杰. 利用小球藻构建微生物燃料电池[J]. , 2009, 9(1): 133-137. |
[8] | 尹良鸿吴晓玉廖鲜艳堵国成陈坚. 产朊假丝酵母发酵生产谷胱甘肽的氨基酸添加策略[J]. , 2008, 8(2): 333-338. |
[9] | 林晨露;李强;刘亚飞;王波. 产GL-7ACA酰化酶基因工程菌的高活性表达[J]. , 2008, 8(1): 140-143. |
[10] | 刘慧娟;华兆哲;堵国成;刘立明;陈坚. 芽孢杆菌发酵生产碱性果胶酶的温度控制策略[J]. , 2007, 7(4): 786-789. |
[11] | 魏明;姜绍通;罗建平. 霍山石斛类原球茎在气升式反应器中补料培养合成多糖[J]. , 2007, 7(2): 375-379. |
[12] | 李兴武;李元广;沈国敏;杨东. 普通小球藻异养-光自养串联培养的培养基[J]. , 2006, 6(2): 277-280. |
[13] | 韩兴梅;李元广;魏晓东;孙勇如;王义琴. 氮源对转基因小球藻异养生长及兔防御素表达的影响[J]. , 2004, 4(1): 16-21. |
[14] | 佟晓冬;孙彦. 核壳型钕铁硼琼脂糖载体的制备及其膨胀床特性[J]. , 2002, 2(3): 0-0. |
[15] | 陈恒志;李洪钟. 高密度循环流化床研究现状及展望[J]. , 2002, 2(2): 0-0. |
PDF全文下载地址:
http://www.jproeng.com/CN/article/downloadArticleFile.do?attachType=PDF&id=3058