姓　　名：赵建国
学　　科：发育生物学
电话/传真：+86-/
电子邮件：zhaojg@ioz.ac.cn, zhaojervis@hotmail.com
通讯地址：北京市朝阳区北辰西路1号院5号
中国科学院动物研究所干细胞与生殖生物学国家重点实验室 100101
更多信息：大动物遗传修饰研究组

简历介绍：
　　赵建国，中国科学院动物研究所干细胞与生殖生物学国家重点实验室研究员，大动物遗传修饰研究组组长, 博士研究生导师，中国科学院大学岗位教授，中国科技大学，安徽大学兼职博士研究生导师。2019年获得国家****科学基金资助。
　　主要研究方向为基于正向遗传学和反向遗传学研究体系的猪功能基因解析和突变体创制，致力于为生物医学研究和临床前转化提供理想的大动物疾病模型，同时也可以为猪的经济性状改良和品系培育提供新的手段和解决思路。具体研究方向为：1. 通过化学诱变进行猪全基因组水平的随机突变和表型分析，建立突变家系，筛选功能基因和培育育种新材料；2. 通过基因组编辑（Genome Editing, GE）技术进行猪的基因组遗传修饰进而改善猪的农业生产性状或构建生物医学研究的大动物模型；3. 猪早期胚胎发育的表观遗传调控和体细胞核移植。率先建立了基于化学诱变的猪正向遗传学研究体系，筛选到一系列与生长、发育相关的突变体，鉴定了包含DUXO2，MITF和ABCA12等在内的12个猪重要功能基因和突变位点，不但为进一步培育猪新品种提供了育种材料和分子标记，也促进了猪在生物医学中的应用。通过核酸酶介导的多维度高效猪基因编辑系统，创制了多种类型的基因编辑猪；解析了藏猪和民猪的抗寒遗传调控机制，创制了低脂、高瘦肉率和抗寒的UCP1定点敲入基因编辑猪， FIX猪乳腺生物反应器等多个育种素材，为猪的功能基因研究和基因编辑技术介导的遗传改良提供了技术保障和理论指导；发现大幅度提高猪克隆效率的新方法。近5年以通讯作者（含共同）身份在PNAS、Blood、EMBO Mol Med、eLife、Natl Sci Rev、J Mol Cell Biol、Biol Reprod等期刊发表SCI论文多篇。担任“Sci Rep”、“J Mol Cell Biol”、“J Integr Agr”等杂志编委会委员。担任中国畜牧兽医学会动物遗传育种学分会及中国动物学会生殖生物学分会等学会常务理事。
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承担科研项目情况：
代表论著：
Wang X^§, Cao C^§, Zhang Y, Jia Q, Zheng Q, Zhang H, Song R, Li Y, Luo A, Hong Q, Qin G, Yao J, Zhang N, Wang Y, Zhou Q, Zhao JG^*. A harlequin ichthyosis pig model with a novel ABCA12 mutation can be rescued by acitretin treatment. J Mol Cell Biol, 2019, 11 (12), 1029-1041. (Cover story)
Zhao JG^§, Lai L, Ji W, Zhou Q^*. Genome editing in large animals: Current status and future prospects. Natl Sci Rev, 2019, 6: 402-420. (Invited review)
Cao C^§, Zhang Y^§, Jia Q, Wang X, Zheng Q, Zhang H, Song R, Li Y, Luo A, Hong Q, Qin G, Yao J, Zhang N, Wang Y, Wang H,Zhou Q, Zhao JG^*. An exonic splicing enhancer mutation in DUOX2 causes aberrant alternative splicing and severe congenital hypothyroidism in Bama pigs. Dis Model Mech, 2019, 12: dmm036616.
Zhang Y^§, Hong Q, Cao C, Yang L, Li Y, Hai T, Zhang H, Zhou Q, Sui R^*, Zhao J^*. A novel porcine model reproduces human oculocutaneous albinism type II. Cell discovery, 2019, 5: 48.
Zhang H^§, Huang J^§, Li Z^§, Qin G, Zhang N, Hai T, Hong Q, Zheng Q, Zhang Y, Song R, Yao J, Cao C, Zhao JG^*, Zhou Q^*. Rescuing ocular development in an anophthalmic pig by blastocyst complementation. EMBO Mol Med. 2018;10(12). pii: e8861.
Wang Y^§*, Huang JJ, Zhao JG^*. Gene engineering in swine for agriculture. J Integr Agr. 2017; 16(12): 2792-2804.
Hai T^§, Guo W^§, Yao J^§, Cao C, Luo A, Qi M, Wang X, Wang X. Huang J, Zhang Y, Wang D, Shang H, Hong Q, Zhang R, Jia Q, Zheng Q, Qin G, Li Y, Zhang T, Jin W, Chen ZY, Wang H, Zhou Q, Meng A, Wei H^*, Yang S^*, Zhao JG^*. Creation of miniature pig model of human Waardenburg syndrome type 2A by ENU mutagenesis. Human genetics. 2017; 136:1463-1475.
Zheng Q^§, Lin J^§, Huang J^§, Zhang H, Zhang R, Zhang X, Cao C, Hambly C, Qin G, Yao J, Song R, Jia Q, Wang X, Li Y, Zhang N, Piao Z, Ye R, Speakman JR, Wang H, Zhou Q, Wang Y^*, Jin W^*, Zhao JG^*. Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decrease fat deposition and improve thermogenic capacity. Proc Natl Acad Sci U S A. 2017; 114(45): E9474-E9482.
Zhang Y^§, Xue Y^§, Cao C^§，Huang J, Hong Q, Hai T, Jia Q, Wang X, Qin G, Yao J, Wang X, Zheng Q, Zhang R, Li Y, Luo A, Zhang N, Shi G, Wang Y, Ying H, Liu Z, Wang H, Meng A, Zhou Q, Wei H^*, Liu F^*, Zhao JG^*. Thyroid hormone regulates hematopoiesis via the TR-KLF9 axis. Blood. 2017; 130(20):2161-2170.
Huang J^§, Wang Y, Zhao J^*. CRISPR editing in biological and biomedical investigation. J Cell Physiol. 2018 May;233(5):3875-3891
Hai T^§, Cao C^§, Shang H^§, Guo W^§, Mu Y^§, Yang S, Zhang Y, Zheng Q, Zhang T, Wang X, Liu Y, Kong Q, Li K, Wang D, Qi M, Hong Q, Zhang R, Wang X, Jia Q, Wang X, Qin G, Li Y, Luo A, Jin W, Yao J, Huang J, Zhang H, Li M, Xie X, Zheng X, Guo K, Wang Q, Zhang S, Li L, Xie F, Zhang Y, Weng X, Yin Z, Hu K, Cong Y, Zheng P, Zou H, Xin L, Xia J, Ruan J, Li H, Zhao W, Yuan J, Liu Z, Gu W, Li M, Wang Y, Wang H^*, Yang S^*, Liu Z^*, Wei H^*, Zhao JG^*, Zhou Q^*, Meng A^*. Pilot study of large-scale production of mutant pigs by ENU mutagenesis. Elife. 2017 Jun 22; 6. pii: e26248.
Lin J^§, Cao C^§, Tao C^§, Ye R, Dong M, Zheng Q, Wang C, Jiang X, Qin G, Yan C, Li K, Speakman JR, Wang Y^*, Jin W^*, Zhao JG^*. Cold adaptation in pigs depends on UCP3 in beige adipocytes. J Mol Cell Biol. 2017 Oct 1; 9(5):364-375.
Jia Q^§, Cao C^§, Tang H, Zhang Y, Zheng Q, Wang X, Zhang R, Wang X, Luo A, Wei H, Meng A, Zhou Q, Wang H^*, Zhao JG^*. A 2-bp insertion (c.67_68insCC) in MC1R causes recessive white coat color in Bama miniature pigs. J Genet Genomics. 2017 Apr 20; 44(4):215-217.
Yao J^§, Huang J, Zhao JG^*. Genome editing revolutionize the creation of genetically modified pigs for modeling human diseases. Hum Genet. 2016 Sep;135(9):1093-105.
Wang X^§, Cao C^§, Huang J^§, Yao J, Hai T, Zheng Q, Wang X, Zhang H, Qin G, Cheng J, Wang Y, Yuan Z, Zhou Q, Wang H^*, Zhao JG^*. One-step generation of triple gene targeted pigs using CRISPR/Cas9 system. Sci Rep. 2016; 6:20620.
Huang J^§, Zhang H, Yao J, Qin G, Wang F, Wang X, Luo A, Zheng Q, Cao C, Zhao JG^*. BIX-01294 increases pig cloning efficiency by improving epigenetic reprogramming of somatic cell nuclei. Reproduction. 2016 Jan; 151(1):39-49.
Zhao JG^§. , Xu WJ, Ross JW., Walters EM., Butler SP., Whyte JJ., Kelso L., Fatemi M, Vanderslice NC., Giroux K., Spate LD., Samuel MS., Murphy CN., Wells KD., Masiello NC., Prather RS^*. & Velander WH^*. Engineering protein processing of the mammary gland to produce abundant hemophilia B therapy in milk. Sci Rep. 2015 Sep 21; 5: 14176.
Wang X^§, Zhou J^§, Cao C^§, Huang J, Hai T, Wang Y, Zheng Q, Zhang H, Qin G, Miao X, Wang H, Cao S^*, Zhou Q^*, Zhao JG^*. Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs. Sci Rep. 2015 Aug 21; 5: 13348.
Zhao W^§, Wang Y^§, Liu S, Huang J, Zhai Z, He C, Ding J, Wang J, Wang H, Fan W, Zhao JG^*, Meng H^*.The dynamic distribution of porcine microbiota across different ages and gastrointestinal tract segments. PLoS One. 2015, 10(2): e**.
Huang J^§, Zhang H, Wang X, Dobbs KB, Yao J, Qin G, Whitworth K, Walters EM, Prather RS^*, Zhao JG^*. Impairment of Preimplantation Porcine Embryo Development by Histone Demethylase KDM5B Knockdown Through Disturbance of Bivalent H3K4me3-H3K27me3 Modifications. Biol Reprod. 2015, 92(3):72. (^*Co-corresponding Author)
Yao J^§, Huang JJ, HaiTang , Wang XL, Qin GS, Zhang HY, Wu R, Xi J. J, Yuan Z, Zhao JG^*. Efficient bi-allelic gene knockout and site-specific knock-in mediated by TALENs in pigs. Sci Rep. 2014, 4:6926. (^*Corresponding Author)
Wang YP^§, Qi ST, Wei Y, Ge ZJ, Chen L, Hou Y, Ouyang YC, Schatten H, Zhao JG^*, Sun QY^*.Knockdown of UCHL5IP causes abnormalities in γ-tubulin localisation, spindle organisation and chromosome alignment in mouse oocyte meiotic maturation. Reprod Fertil Dev. 2013; 25(3):495-502. (^*Co-corresponding Author)
Whyte JJ^§,Zhao JG^§, Samuel M, Laughlin HM, Prather RS^*, Gene Targeting with Zinc Finger Nucleases to Produce Cloned eGFP Knockout Pigs. Mol Reprod Dev. 2011, 78(1):2.
Zhao JG^§, Whyte JJ, Prather RS^*. The effect of epigenetic regulation on somatic cell nuclear transfer. Cell. Cell Tissue Res. 2010, 341(1):13-21.
Zhao JG^§, Hao Y, Ross JW, Spate LD, Walters EM, Samuel MS, Rieke A, Murphy CN, Prather RS^*. Histone Deacetylase Inhibitors Improve In Vitro and In Vivo Developmental Competence of Somatic cell Nuclear Transfer Porcine Embryos.Cloning and stem cells,2010,12(1):75-83.
Ross JW^§, Prather RS, Whyte JJ,Zhao JG^*.Cloning and transgenics: progress and new approaches.Invited review by Centre for Agricultural Bioscience International,2009, 4, No. 38.
Zhao J^§, Ross JW, Hao Y, Spate LD, Walters EM, Samuel MS, Rieke A, Murphy CN, Prather RS^*. Significant Improvement in Cloning Efficiency of an Inbred Miniature Pig by Histone Deacetylase Inhibitor Treatment after Somatic Cell Nuclear Transfer. Biol Reprod. 2009, 81: 525-530.
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