Mingjie Zheng
Kechao Weng
Yumei Hao
Yao Zhou
Yuchen Lin
Feng Gao
Ziqi Kou
Shoji Kawamura
Ke Yao
Pinglong Xu
Jinghai Chen
Jian Zou
aEye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China
bThe Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China
cDepartment of Cardiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China
dDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba 277-8562, Japan
eZhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310058, China
fMOE Laboratory of Biosystems Homeostasis & Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute, Zhejiang University, Hangzhou 310058, China
More InformationCorresponding author: E-mail address: jianzou@zju.edu.cn (Jian Zou)
Publish Date:2021-01-20
Abstract
Abstract
Although the?unique organization of vertebrate cone mosaics was first described long ago, both their underlying molecular basis and physiological significance are largely unknown. Here, we demonstrate that Crumbs proteins, the key regulators of epithelial apical polarity, establish the planar cellular polarity of photoreceptors in zebrafish. Via heterophilic Crb2a-Crb2b interactions, the apicobasal polarity protein Crb2b restricts the asymmetric planar distribution of Crb2a in photoreceptors. The planar polarized Crumbs proteins thus balance intercellular adhesions and tension between photoreceptors, thereby stabilizing the geometric organization of cone mosaics. Notably, loss of Crb2b in zebrafish induces a nearsightedness-like phenotype in zebrafish accompanied by an elongated eye axis and impairs zebrafish visual perception for predation. These data reveal a detailed mechanism for cone mosaic homeostasis via previously undiscovered apical-planar polarity coordination and propose a pathogenic mechanism for nearsightedness.Keywords: Crumbs proteins,
Apical polarity,
Planar cellular polarity,
Cone mosaics,
Nearsightedness,
Visual perception
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