Chen-Jun Guo
Huan-Huan Hu
Jiale Zhong
Qianqian Sun
Dandan Liu
Shuang Zhou
Chia Chun Chang
Ji-Long Liu
aSchool of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China
bInstitute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China
cUniversity of Chinese Academy of Sciences, Beijing, 100049, China
diHuman Institute, ShanghaiTech University, Shanghai, 201210, China
More InformationCorresponding author: E-mail address: liujl3@shanghaitech.edu.cn (Ji-Long Liu)
Received Date: 2019-10-22
Accepted Date:2019-11-10
Rev Recd Date:2019-11-07
Available Online: 2019-11-29 Publish Date:2019-11-20
Abstract
Abstract
Intracellular compartmentation is a key strategy for the functioning of a cell. In 2010, several studies revealed that the metabolic enzyme CTP synthase (CTPS) can form filamentous structures termed cytoophidia in prokaryotic and eukaryotic cells. However, recent structural studies showed that CTPS only forms?inactive product-bound filaments in bacteria while forming active substrate-bound filaments in eukaryotic cells. In this study, using negative staining and cryo-electron microscopy, we demonstrate that Drosophila CTPS, whether in substrate-bound or product-bound form, can form filaments. Our results challenge the previous model and indicate that substrate-bound and product-bound filaments can coexist in the same species. We speculate that the ability to switch between active and inactive cytoophidia in the same cells provides an additional layer of metabolic regulation.Keywords: CTP synthase,
Cytoophidium,
Cryo-EM
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