Xia Wang
Da Shen
Jin Sun
Huan-Huan Qiao
Fang Wang
Lu-Ping Liu
Jian-Quan Ni
aGene Regulatory Lab, School of Medicine, Tsinghua University, Beijing, 100084, China
bTsinghua University-Peking University Joint Center for Life Sciences, Beijing, 100084, China
cDepartment of Genetics, Harvard Medical School, Boston, MA, 02115, USA
dHoward Hughes Medical Institute, Boston, MA, 02115, USA
eTsingdao Advanced Research Institute, Tongji University, Qingdao, 266000, China
More InformationCorresponding author: E-mail address: nijq@mail.tsinghua.edu.cn (Jian-Quan Ni)
Received Date: 2018-11-19
Accepted Date:2019-03-12
Rev Recd Date:2019-02-27
Available Online: 2019-04-17 Publish Date:2019-04-20
Abstract
Abstract
Gene expression regulation, including loss-of-function and gain-of-function assays, is a powerful method to study developmental and disease mechanisms. Drosophila melanogaster is an ideal model system particularly well-equipped with many genetic tools. In this review, we describe and discuss the gene expression regulation techniques recently developed and their applications, including the CRISPR/Cas9-triggered heritable mutation system, CRISPR/dCas9-based transcriptional activation (CRISPRa) system, and CRISPR/dCas9-based transcriptional repression (CRISPRi) system, as well as the next-generation transgenic RNAi system. The main purpose of this review is to provide the fly research community with an updated summary of newly developed gene expression regulation techniques and help the community to select appropriate methods and optimize the research strategy.Keywords: Genome editing system,
CRISPRa,
CRISPRi,
Transgenic RNAi system
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