Wen Zhao
Xiaobo Zhu
Chengdong Zou
Junjie Yin
Mawsheng Chern
Xiaogang Zhou
Heng Ying
Xin Jiang
Yongzhen Li
Haicheng Liao
Mengping Cheng
Weitao Li
Min He
Jing Wang
Jichun Wang
Bingtian Ma
Jirui Wang
Shigui Li
Lihuang Zhu
Xuewei Chen
aState Key Laboratory of Crop Genetics of Disease Resistance and Disease Control, State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Rice Research Institute, Sichuan Agricultural University, Sichuan 611130, China
bDepartment of Plant Pathology, University of California, Davis, CA 95616, USA
cTriticeae Research Institute, Sichuan Agricultural University, Sichuan 611130, China
dState Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
More InformationCorresponding author: E-mail address: lhzhu@genetics.ac.cn (Lihuang Zhu);E-mail address: xwchen@sicau.edu.cn (Xuewei Chen)
Received Date: 2018-06-08
Accepted Date:2018-10-18
Rev Recd Date:2018-09-11
Available Online: 2018-12-31 Publish Date:2018-12-20
Abstract
Abstract
Map-based cloning of plant disease resistance (R) genes is time-consuming. Here, we reported the isolation of blast R gene Pid4 using comparative transcriptomic profiling and genome-wide sequence analysis. Pid4 encodes a coiled-coil nucleotide-binding site leucine-rich repeat (CC-NBS-LRR) protein and is constitutively expressed at diverse developmental stages in the rice variety Digu. The Pid4 protein is localized in both the nucleus and cytoplasm. Introduction of Pid4 into susceptible rice cultivars confers race-specific resistance to leaf and neck blast. Amino acid sequence comparison and blast resistance spectrum tests showed that Pid4 is a novel R gene, different from the previously reported R genes located in the same gene cluster. A Pid4 Indel marker was developed to facilitate the identification of Pid4 in different rice varieties. We demonstrated that a plant R gene can be quickly isolated using transcriptomic profiling coupled with genome-wide sequence analysis.Keywords: Blast disease,
Genome-wide sequence analysis,
Resistance,
Rice,
Transcriptome
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