Yaoju Tan
Julius Mugweru
Md Mahmudul Islam
H.M. Adnan Hameed
Shuai Wang
Zhili Lu
Changwei Wang
Xinjie Li
Shouyong Tan
Jianxiong Liu
Tianyu Zhang
aState Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
bUniversity of Chinese Academy of Sciences, Beijing 100049, China
cState Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital, Guangzhou 510095, China
dDepartment of Biological Sciences, University of Embu, P.O Box 6 -60100, Embu, Kenya
More InformationCorresponding author: E-mail address: ljxer64@qq.com (Jianxiong Liu);E-mail address: zhang_tianyu@gibh.ac.cn (Tianyu Zhang)
Available Online: 2018-06-18 Publish Date:2018-06-20
Abstract
Abstract
Mycobacterium tuberculosis, a Gram-positive bacterium of great clinical relevance, is a lethal pathogen owing to its complex physiological characteristics and development of drug resistance. Several molecular genetic tools have been developed in the past few decades to study this microorganism. These tools have been instrumental in understanding how M.?tuberculosis became a successful pathogen. Advanced molecular genetic tools have played a significant role in exploring the complex pathways involved in M.?tuberculosis pathogenesis. Here, we review various molecular genetic tools used in the study of M.?tuberculosis. Further, we discuss the applications of clustered regularly interspaced short palindromic repeat interference (CRISPRi), a novel technology recently applied in M.?tuberculosis research to study target gene functions. Finally, prospective outcomes of the applications of molecular techniques in the field of M.?tuberculosis genetic research are also discussed.Keywords: Molecular genetic tools,
Gram-positive,
Recombineering,
Drug resistance
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