中文关键词:CO_3^2-比率型荧光探针实际水样MCF-7细胞 英文关键词:CO_3^2-, ratiofluorescent probe, actualwater, MCF-7 cells 基金项目:国家自然科学基金项目(21878023,U1608222)和辽宁****支持计划项目资助

作者	单位	E-mail
王鑫a	a渤海大学化学与材料工程学院	944470860@qq.com
李方霁a	a渤海大学化学与材料工程学院
钟克利a	a渤海大学化学与材料工程学院
侯淑华a	a渤海大学化学与材料工程学院
燕小梅b	大连医科大学检验医学院
边延江a	a渤海大学化学与材料工程学院 锦州121013 b大连医科大学检验医学院 大连116044	bianyanjiang@126.com
汤立军a	a渤海大学化学与材料工程学院

摘要点击次数:128 全文下载次数:0 中文摘要: 本文设计开发了一种以2,6-二甲酰基对甲苯酚为母体的新型荧光探针HMI,可用于高效识别EtOH-H₂O (8/2, v/v, HEPES 10 mM, pH =7.4)体系中的CO_3^2-。HMI在660 nm处显示发射带,加入CO_3^2-后,在600 nm的等吸收点激发时,原来在660 nm处的荧光淬灭,而以540 nm为中心的新发射带荧光显着增加,为比率型荧光探针。HMI对CO_3^2-表现出高选择性且具有较强的抗干扰能力。此外,荧光探针HMI对CO_3^2-荧光响应的检测限较低,可达到3.938×10^_-6 M。更具有意义的是,HMI探针对CO_3^2-的检测能够在实际水样中起到很好的应用,而且细胞成像研究表明,HMI可用于活体MCF-7细胞中CO_3^2-的成像。 英文摘要: In this work, we designed and developed a new fluorescent probe HMI using 2,6-diformyl-p-cresol as the precursor, which could be used to efficiently identify CO_3^2- in EtOH-H₂O (8/2, v/v, HEPES 10 mM, pH =7.4). The HMI showed an emission band at 660 nm, when excited at the absorption point of 600 nm, the original emission at 660 nm decreased and the new emission band centered at 540 nm increased significantly after adding CO3^_2-, which showed that HMI was a ratio fluorescent probe. HMI showed high selectivity to CO_3^2- and had strong anti-interference ability. In addition, the detection limit of the fluorescence response of the fluorescent probe HMI to CO_3^2-is low, which could reach 3.939×10^_-6 M. More meaningfully, the detection of CO_3^2-by the HMI probe could play a good application in actual water samples and cell imaging studies indicate that HMI is applicable to image CO_3^2- in living MCF-7 cells. 查看全文查看/发表评论下载PDF阅读器 相关附件：版权转让声明书附件1 -->